HPLC-ELSD测定复方参芪软胶囊中黄芪甲苷的含量  

Content Determination of Astragaloside in Compound Shenqi Soft Capsule by HPLC-ELSD

作　　者：王春艳[1] 杨建春[1] 于庆坤[1] 张琦[1] 潘卫三[2] 

机构地区：[1]唐山市妇幼保健院,河北唐山063000 [2]沈阳药科大学药学院,沈阳110016

出　　处：《中国现代应用药学》2010年第9期846-848,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立以高效液相色谱-蒸发光散射检测法测定复方参芪软胶囊中黄芪甲苷含量的方法。方法色谱柱为AgilentHypersil ODS,流动相为乙腈-水（35∶65）,流速为1.0 mL.min-1,进样量为20μL;蒸发光散射检测器条件：载气（压缩空气）压力为0.32 MPa,载气流量：2.6 L.min-1,漂移管温度为108℃。结果黄芪甲苷进样量的线性范围为0.402 4~4.024 0μg（r=0.999 8）;平均回收率为100.02%（RSD=1.41%）。结论该方法准确度高、专属性强,可用于该制剂中黄芪甲苷的含量测定。OBJECTIVE To establish an HPLC-ELSD method for the determination of astragaloside in compound shenqi soft capsule.METHODS The samples were determined by HPLC-ELSD on an Agilent Hypersil ODS column using acetonitrile-water（35∶65） as the mobile phase at a flow rate of 1.0 mL.min-1.The injection volume was 20 μL.The ELSD conditions were as follows： pressure of compressed air was 0.32 MPa,the flow rate of compressed air was 2.6 L.min-1,drift temperature was 108 ℃.RESULTS The linear range of astragaloside was 0.402 4-4.024 0 μg（r=0.999 8） and the average recovery rate was 100.02%（RSD=1.41%）.CONCLUSION The method is accurate,specific and applicable for the determination of astragaloside in compound shenqi soft capsule.

关 键 词：高效液相色谱-蒸发光散射检测法 复方参芪软胶囊 黄芪甲苷 含量测定 

分 类 号：R917.101[医药卫生—药物分析学] R927.2[医药卫生—药学]