蛋氨酸胆碱对铅暴露大鼠脏器微量元素影响  

Effects of methionine and choline on trace elements in tissues of lead-exposed rats

作　　者：伍凤云[1] 林芬[1] 范广勤[1] 冯昶[1] 闫冀[1] 王红海[1] 黄贇 肖元梅[1] 黎砚书[1] 

机构地区：[1]南昌大学公共卫生学院劳动卫生与毒理教研室,江西南昌330006

出　　处：《中国公共卫生》2010年第10期1242-1243,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30760211);江西省自然科学基金(2009GZY0138);江西省教育厅科技项目(2007110)

摘　　要：目的探讨蛋氨酸胆碱对铅暴露SD大鼠体内微量元素影响。方法将60只SD大鼠随机分为空白对照组、染铅组、蛋氨酸胆碱组、铅+低、高剂量蛋氨酸胆碱组,分别给予铅(饮水)和蛋氨酸胆碱(灌胃),麻醉取脑、肾、肝和骨,采用电感耦合等离子发射光谱法测定组织中铅、铜、铁、锰、锌及钙含量。结果染铅后,脑、肝、肾、骨组织中铅含量明显增高,分别为(0.631±0.188),(1.178±0.354),(12.669±2.682)和(165.621±12.015)μg/g,铅+低、高剂量蛋氨酸胆碱组肝组织铅含量分别降至(0.955±0.299)和(0.637±0.164)μg/g;铅+高剂量蛋氨酸胆碱组肾铅和骨铅含量分别降至(9.789±1.743)和(132.742±17.743)μg/g,明显低于铅对照组(P<0.01);铅对照组脑铁和铜及肝铁含量高于空白对照组,给予蛋氨酸胆碱后,脑铁和铜及肝铁含量下降至空白对照组水平。结论蛋氨酸胆碱可降低肝、肾和骨组织中铅含量,对铜、铁、锰、锌无明显影响。Objective To explore the effects of methionine and choline on contents of microelement in tissues of rats.Methods Sixty weanling SD rats were randomly divided into five groups：normal control group,lead control group,methionine and choline control group,and two lead plus different dose of methionine and choline groups.The rats were given lead（drinking water） and methionine choline（intragastric administration）,respectively.At the end of experiment,the brain,kidney,liver,and bone of the rats were collected.Inductively coupled plasma-atomic emission spectrophotometry（ICP-AES） was adopted to determine the contents of lead（Pb）and other 5 kinds of metal elements（Zn,Cu,Fe,Mn,and Ca） in the brain,kidney,liver,and bone.Results After lead exposure,the lead contents in the brain,liver,kidney,and bone increased significantly（0.631±0.188,1.178±0.354,12.669±2.682,165.621±12.015 μg/g）.The lead contents in liver of two different methionine and choline dose groups were reduced to 0.955±0.299,0.637±0.164 μg/g.The lead contents in kidney and bone of high methionine and choline group were reduced to 9.789±1.743,132.742±17.743 μg/g,significantly lower than that of lead control group（P0.01）.The contents of Fe and Cu in the brain and Fe in the liver of lead control group were higher than those of normal control group and decreased to normal level of control group after methionine and choline treatment.Conclusion Methionine and choline treatment reduce the level of lead in the liver,kidney and bone in lead-exposed rats while the contents of Cu,Fe,Mn,and Zn in the tissues are no significant change.

关 键 词：蛋氨酸 胆碱 铅 微量元素 

分 类 号：R994.6[医药卫生—毒理学]