家系内慢性HBV感染与HLA-Ⅱ类基因多态性  

Association between familial chronic HBV infection and HLA-Ⅱ gene polymorphism

作　　者：谢德胜[1] 张清波[2] 黄建芳[1] 陈常兴[1] 马瑨[1] 黄海滨[1] 度尧[1] 

机构地区：[1]复旦大学附属上海市第五人民医院感染科,上海200240 [2]复旦大学附属华山医院

出　　处：《中国公共卫生》2010年第10期1261-1263,共3页Chinese Journal of Public Health

基　　金：上海市闵行区自然科学研究项目(2007MH020)

摘　　要：目的探讨家系内慢性乙型肝炎病毒感染与人类白细胞抗原-Ⅱ(HLA-Ⅱ)类基因多态性的关系及可能的原因。方法采用聚合酶链反应序列特异性引物(PCR/SSP)对(37个)慢性乙型肝炎病毒感染家系内成员(慢性HBV感染组121人,HBsAg阴性组76人)的人类白细胞抗原(HLA)DQA1和DQB1等位基因多态性进行分析;并从外周血中分离单个核细胞,在培养基中诱导培养为树突状细胞(DC),采用混合淋巴细胞反应检测DC刺激淋巴细胞增殖的能力。结果 HLA-DQA1 0501和DQB1 0301,DQB1 0201在慢性乙型肝炎病毒(HBV)感染组的等位基因频率明显高于HBsAg阴性组;HLA-DQB1 0402和DQB1 0604等位基因频率明显低于HBsAg阴性组;慢性HBV感染组DC刺激淋巴细胞增殖的刺激指数(SI)与HBsAg阴性组比较明显降低(P<0.01);HLA-DQA1 0501、DQB1 0301阳性组DC刺激淋巴细胞增殖指数明显低于阴性组;HLA-DQB1 0402、DQB1 0604阳性组淋巴细胞增殖指数明显高于阴性组。结论家系内HBV慢性感染与HLA-DQA1和DQB1的基因多态性相关,HLA-Ⅱ类基因可通过影响DC刺激淋巴细胞增殖能力发挥免疫调查作用,可能与HBV感染慢性化有关。Objective To study the relationship between familial chronic hepatitis B virus（HBV） infection and human leucocyte antigen（HLA-Ⅱ） class gene polymorphism.Methods HLA-DQA1 and DQB1 allele polymorphism was analyzed among persons with and without chronic HBV infection in 37 families with 121 persons of chronic HBV infection and 76 persons of HBsAg-negative with PCR/SSP.Peripheral blood mononuclear cells were cultured in the medium to induce dendritic cell（DC） and mixed lymphocyte reaction was adopted to detect the capacity of DC for stimulating lymphocyte proliferation.Results The allele frequencies of HLA-DQA1 0501 and DQB1 0301,DQB1 0201 in patients with chronic HBV infection were significantly higher than those of HBsAg-negative group.HLA-DQB1 0402 and DQB1 0604 allele frequency of the patients was significantly lower than that of HBsAg-negative group.The lymphocyte proliferation index stimulation index（SI） in HLA-DQA1 0501,DQB1 0301 positive group was significantly lower than that of negative group（P0.01） and HLA-DQB1 0402 and DQB1 0604 in positive group was significantly higher than that in the negative group.Conclusion Familial chronic HBV infection is closely associated with HLA-DQA1 and DQB1 gene polymorphism.HLA-Ⅱ genes can affect the DC capacity to stimulate lymphocyte proliferation and play a role in immune regulation,and may be related to chronic infection of the HBV.

关 键 词：乙型肝炎病毒 类基因多态性 树突状细胞 淋巴细胞增殖反应 

分 类 号：R512.62[医药卫生—内科学]