苯并[a]芘在大鼠脑组织中分布动态观察  

Disposition of benzo[a]pyrene in brain tissue of rat

作　　者：汤艳[1] 陈承志[2] 蒋学君[3] 涂白杰[2] 

机构地区：[1]泸州医学院公共卫生系环境卫生与劳动卫生教研室,四川泸州646000 [2]重庆医科大学公共卫生学院 [3]四川大学华西公共卫生学院环境卫生教研室

出　　处：《中国公共卫生》2010年第10期1277-1278,共2页Chinese Journal of Public Health

基　　金：国家自然科学资金(30671744)

摘　　要：目的观察[14C]标记苯并[a]芘在大鼠脑内的分布。方法将100只SD大鼠随机分为对照组和实验组,实验组尾静脉注射[14C]标记苯并[a]芘3.7×105Bq/kg,对照组注射等量生理盐水;在注射后1,6,12,24和48 h用光镜放射自显影法观察苯并[a]芘在脑内的分布情况。结果与对照组比较,实验组大鼠脑组织注药后1 h即有苯并[a]芘银粒出现,随着时间的增加,银粒数也逐渐增加,24 h达高峰,48 h明显减少,注射后1,6,12,24,48 h银粒数分别为(17.68±1.79),(22.67±2.15),(25.79±2.55),(32.33±2.78),(18.01±1.68)粒,差异有统计学意义(F=69.67,P<0.01);银粒在脑中分布不均匀,注药后1 h主要集中在海马,6 h主要集中于大脑皮层,12 h则分布于纹状体;各时相脑组织中神经元银粒数明显高于神经胶质细胞(P<0.05)。结论苯并[a]芘在各脑区的分布不均匀,24 h达到高峰,神经元细胞可能是苯并[a]芘靶细胞。Objective To study the dynamic distribution of benzo[a]pyrene in the brain tissue of rat by light microscopic autoradiography.Methods One hundred SD rats were divided into control group and test group.The experimental animals were given a single intravenous injection of 3.7×105 Bq/kg of 14C-benzo[a]pyrene while the same dose of normal saline was given to the control group.The rats were sacrificed at 1,6,12,24,and 48 hours after the administration of radiolabelled benzo[a]pyrene.Light microscopic autoradiography was used to observe the dynamic distribution of benzo[a]pyrene in brain tissue.Results After the administration,the number of silver granules increased along with the time increasing,which reached the peak in 24 hr and decreased sharply in 48 hr（P0.01）.Light microscopic autoradiography showed that benzo[a]pyrene centralized in hippocampus at 1hr,in cerebral cortex at 6 hr,and in corpus striatum at 24 hr after the treatment.The silver granule distribution was more in neuron than that of in neurogliocyte（P0.05）.Conclusion Benzo[a]pyrene distributes unevenly in every region of rat brain and the nerve cell is probaly the target cell of benzo[a]pyrene.

关 键 词：苯并[A]芘 光镜放射自显影 脑组织 

分 类 号：R114[医药卫生—卫生毒理学]