松毛虫质型多角体病毒林间增殖与提取技术初报  

Technology of Field Multiplication and Extraction of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus (DpCPV)

作　　者：余金勇 黄海勇 吴跃开 程绍传 

机构地区：[1]贵州省林业科学研究院 [2]贵州省森林病虫检疫防治站

出　　处：《贵州林业科技》2010年第3期1-5,22,共6页Guizhou Forestry Science and Technology

基　　金：贵州省林业厅黔科合字(2004-01)项目支助;黔科合院所创能[2009]4002

摘　　要：在林间喷施不同浓度的松毛虫质型多角体病毒(CPV)2.8×107PIB/ml、2.8×106PIB/ml、2.8×105PIB/ml15天后,调查结果表明:三种浓度的CPV感染马尾松毛虫后中肠发白率分别达到72.2%、69.2%、20.2%。林间给马尾松毛虫自然种群喷施2.8×106PIB/mlCPV增殖病毒,经济有效。开展了大规模CPV提取试验,提取的CPV制剂多角体达25.2×108PIB/g,病毒提取率6.8%。进行了CPV保存试验,试验表明低温有利于CPV保存。Different concentrations（2.8×107PIB/ml、2.8×106PIB/ml、2.8×105PIB/ml） of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus（DpCPV） were sprayed in the forest,and after 15 days,infection rates of Dendrolimus punctatus larvae were 72.2﹪、69.2﹪、20.2﹪,respectively.This result showed that spraying of DpCPV with concentration of 2.8×106PIB/ml is cost-effective.Furthermore,extraction experiment of DpCPV was conducted and,as a result,25.2×108PIB/g of DpCPV was obtained,with an extraction rate of 6.8﹪.This study also showed that lower temperature is suitable for the preservation of DpCPV.

关 键 词：松毛虫 松毛虫质型多角体病毒 病毒增殖 

分 类 号：S763[农业科学—森林保护学]