反相高效液相色谱法测定血浆中甲氨蝶呤的质量浓度  

Determination of Methotrexate in Plasma by RP-HPLC

作　　者：李碧峰[1] 冯惠平[1] 贾晋蓉[1] 陈宜锋[1] 叶志梅[1] 

机构地区：[1]福建医科大学附属漳州市医院药剂科,福建漳州363000

出　　处：《中国药业》2010年第19期7-8,共2页China Pharmaceuticals

摘　　要：目的建立测定血浆中甲氨蝶呤浓度的反相高效液相色谱法,为临床合理用药提供参考。方法色谱柱采用Agilent TC-C18柱(250 mm×4.6 mm,5μm),流动相为0.025 mol/L磷酸二氢钠溶液(pH为5.41)-甲醇(76∶24,V/V),流速1.0 mL/min,检测波长313 nm,柱温35℃,样品经高氯酸沉淀蛋白后直接进样。结果血浆中甲氨蝶呤的质量浓度在0.05～5μg/mL和5～100μg/mL范围内与峰面积线性关系良好,r1=0.999 9,r2=0.999 8,平均回收率分别为98.06%和98.79%,最低定量质量浓度为0.05μg/mL,日内及日间精密度的RSD均小于4%(n=3)。结论所用方法简便快速、准确可靠,灵敏度高,重现性好,线性范围宽,成本低,适于甲氨蝶呤的血药浓度监测。Objective To set up a rapid RP-HPLC method to determine the plasma concentration of methotrexate（MTX）.Methods The analytical column was Agilent TC-C18（250 mm × 4.6 mm,5 μm）.The mobile phase was 0.025 mol /L NaH2PO4（pH = 5.41）-methanol（76 ∶ 24,V/V）.The flow rate was 1.0 mL/min.The detection wavelength was 313 nm.The column temperature was set at 35 ℃.The plasma samples were deposited down the protein with 10% HClO4.Results The linear relation was in the range of 0.05-5 μg/mL（r1 = 0.999 9） and the range of 5-100 μg/mL（r2 = 0.999 8）,respectively.The average recovery rates were 98.06% and 98.79%,respectively.The measurable lowest concentration was 0.05 μg/mL.RSD with intra-day and inter-day was less than 4%.Conclusion The established method is simple,accurate,sensitive and reproducible with large-scale of detection range,which is suitable for monitoring the blood concentration of methotrexate.

关 键 词：反相高效液相色谱法 甲氨蝶呤 血药浓度监测 

分 类 号：R969.1[医药卫生—药理学] R979.1[医药卫生—药学]