高效液相色谱法测定排石康颗粒剂中齐墩果酸含量  

Determination of Oleanolic Acid in Paishikang Granules by HPLC

作　　者：任贻军[1] 周菁丽[1] 李纪元[1] 李华荣[1] 高逢喜[1] 周海[2] 

机构地区：[1]湖北省荆州市中心医院,湖北荆州434020 [2]郧阳医学院药学系,湖北十堰442000

出　　处：《中国药业》2010年第19期31-32,共2页China Pharmaceuticals

摘　　要：目的建立测定排石康颗粒剂中齐墩果酸含量的高效液相色谱法。方法采用Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.4%磷酸溶液(85∶15),流速1 mL/min,检测波长208 nm。结果齐墩果酸质量浓度在2.4～38.4μg/mL范围内与峰面积线性关系良好,r=0.999 8(n=6),平均回收率为98.97%,RSD=1.97%(n=6)。结论所用方法简便灵敏、准确、重复性好,可作为排石康颗粒剂的质量控制方法。Objective To establish the determination method for oleanolic acid in Paishikang Granules by HPLC.Methods The Eclipse XDB-C18 column（250 mm × 4.6 mm,5 μm） was selected and the mobile phase consisted of acetonitrile-0.4% phosphoric acid（85∶15） with the flow rate of 1 mL/min.The detection wavelength was 208 nm.Results Oleanolic acid had good linearity in the range of 2.4-38.4 μg/mL（r = 0.999 8）,the average recovery rate was 98.97%,RSD = 1.97%（n = 6）.Conclusion The method is simple and accurate with good reproducibility,which can be much suitable for the determination of oleanolic acid in Paishikang Granules.

关 键 词：排石康颗粒剂 齐墩果酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]