固相萃取-高效液相色谱法测定马尾松组织中内源激素  

Determination of Endogenous Hormones in (Pinus massoniana Lamb) Using Solid Phase Extraction-High Performance Liquid Chromatography

作　　者：王水良[1] 王平[1] 王趁义[2] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037 [2]湖州师范学院化学系,浙江湖州313000

出　　处：《分析科学学报》2010年第5期547-550,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.30771696)

摘　　要：建立了固相萃取-高效液相色谱法(SPE-HPLC)测定马尾松组织中内源激素赤霉素(GA3)、吲哚乙酸(IAA)、玉米素核苷(ZR)和脱落酸(ABA)的分析方法。采用WatersC18反相柱(250×4.6mmi.d.,5μm),以体积比为45∶54∶1的甲醇-水-乙酸溶液为流动相,流速1.0mL/min;进样量20μL;检测波长254nm;外标法定量测定。选用Sep-PakC18固相萃取小柱富集内源激素,再经流动相洗脱预处理。4种内源激素在1.0～3.0μg/g添加范围内的回收率为88.4%～108.3%,GA3、IAA、ZR和ABA检出限依次为0.05μg/g、0.02μg/g、0.03μg/g和0.01μg/g。An analytical method based on solid phase extraction-high performance liquid chromatography（SPE-HPLC）has been established for the determination of endogenous hormones [gibberellin acid（GA3）,indole-3-acetic acid（IAA）,zeatin ribode（ZR）,abscisic acid（ABA）] in masson pine（Pinus massoniana Lamb）.Separation was performed on a Waters C18 reversed-phase column（250 mm×4.6 mm i.d.,5 μm）,using a mixture of methanol,water and acetic acid（45∶54∶1,volume ratio）as mobile phase at a flow rate of 1.0 mL/min.The ultraviolet detection was set at 254 nm,injection volume was 20 μL,and the quantitive ana1ysis of external standard calibration curves was used.The samples were purified by a Sep-Pak C18 solid phase extraction column,and then eluted by the mobile phase.The recoveries of the 4 endogenous hormones in the spiked samples at levels of 1.0~3.0 μg/g were in the range of 88.4%~108.3%.The limits of detection were 0.05 μg/g for GA3,0.02 μg/g for IAA,0.03 μg/g for ZR and 0.01 μg/g for ABA,respectively.The results indicate that the proposed method is easy,time saving and sensitive.

关 键 词：固相萃取 高效液相色谱法 内源激素 马尾松 

分 类 号：O657.72[理学—分析化学]