通心络超微粉对局灶性脑缺血大鼠神经行为学和脑梗死面积的影响  

The interventional effects of Tongxinluo super-micropowder on infarction area and neurological behavior of focal cerebral ischemia

作　　者：常丽萍 张秋燕 韩建科 张东皎 王宏涛 贾振华 

机构地区：[1]河北以岭医药研究院,石家庄050035

出　　处：《疑难病杂志》2010年第10期727-730,共4页Chinese Journal of Difficult and Complicated Cases

基　　金：国家重点基础研究发展计划(国家973计划)资助项目(No.2005CB523301);国家十一五"重大新药创制项目"(No.2009ZX09313-003)

摘　　要：目的探讨通心络超微粉对局灶性脑缺血大鼠神经行为学和脑梗死面积的干预作用。方法利用开颅结扎法阻断大鼠一侧大脑中动脉制作局灶性脑缺血模型(MCAO),筛选后随机分为假手术组、模型组、通心络大剂量组、通心络中剂量组、通心络小剂量组、尼莫地平组,灌胃给药3 d、7 d、14 d后,积分法测定MCAO大鼠神经行为学评分,氯化三苯基四氮唑(TTC)染色法测定大脑梗死面积。结果给药3 d后,通心络大剂量组、尼莫地平组与模型组比较差异有统计学意义(P<0.05),通心络大剂量组与尼莫地平组比较差异无统计学意义(P>0.05),通心络中、小剂量组与模型组比较差异无统计学意义(P>0.05)。给药7d后,通心络大剂量组、通心络中剂量组、尼莫地平组与模型组比较差异均有统计学意义(P<0.05,P<0.01),且通心络大剂量组优于通心络小剂量组(P<0.05);给药14 d后,通心络大剂量组、通心络中剂量组、通心络小剂量组、尼莫地平组与模型组比较差异均有统计学意义(P<0.05,P<0.01),且通心络大剂量组优于通心络中、小剂量组及尼莫地平组(P<0.05)。结论通心络能够显著改善局灶性脑缺血模型大鼠神经功能障碍,缩小脑梗死面积,具有显著的神经保护作用。Objective To explore the interventional effects of Tongxinluo super-micropowder on infarction area and neurological behavior of focal cerebral ischemia.Methods Using craniotomy ligation a side of the middle cerebral artery established the model of focal cerebral ischemia（MCAO）,after filtration male SD rats were randomly divided into sham-operation group,model control group,TXL-H,TXL-M,TXL-L and nimodipine.After 3 d、7d、14 d,the neurological outcomes were scored.The infarct size of brain was measured by TTC staining.Results After 3d,compared with the model control group,the score of neurological symptom and the infarct size of brain were statistically significant in TXL-H and nimodipine（P0.05）.TXL-H and nimodipine were not statistically significant （P0.05）.TXL-M,TXL-L and model control group were not statistically significant（P0.05 ）;After 7d,compared with the model control group,TXL-H,TXL-M,nimodipine were statistically significant（P0.05,P0.01）.TXL-H was better than TXL-L.After 14d,compared with the model control group,TXL-H,TXL-M,TXL-L, nimodipine were statistically significant（P0.05,P0.01）.TXL-H was better than either of TXL-M,TXL-L or nimodipine（P0.05）.Conclusion Tongxinluo super-micropowder can significantly improve the rats of focal cerebral ischemia in neurological deficits and infarction area.Tongxinluo showed obvious protective effects on focal cerebral ischemia in rats,but the mechanism need further study.

关 键 词：神经行为学 脑缺血 局灶性 通心络超微粉 大鼠 

分 类 号：R285.5[医药卫生—中药学]