Chitosan对红豆杉PAL及Taxol合成的影响  

Effects of Chitosan on the Improvement of Taxol Biosynthesis and Phenylalanine Ammonia Lyase Activity in Taxus Chinensis Suspension Cultures

作　　者：张长河[1] 余龙江[1] 梅兴国[1] 李亚莉 

机构地区：[1]华中理工大学生命科学与技术学院

出　　处：《华中理工大学学报》1999年第4期104-106,共3页Journal of Huazhong University of Science and Technology

基　　金：国家科委"八五"资助;"九五"重点科技攻关资助

摘　　要：研究了Ｃｈｉｔｏｓａｎ（聚氨基葡糖）对红豆杉悬浮培养细胞ＰＡＬ（苯丙氨酸解氨酶）活性及Ｔａｘｏｌ（紫杉醇）生物合成的影响．种胚来源的红豆杉细胞培养在改良ＭＳ液体培养基中，于培养的第１８ｄ添加不同浓度的Ｃｈｉｔｏｓａｎ．Ｃｈｉｔｏｓａｎ浓度为１００～１０００ｍｇ·Ｌ－１时对红豆杉细胞ＰＡＬ活性有明显的诱导作用，且诱导作用随浓度的增加而增强，在诱导作用下ＰＡＬ活性在１６ｈ达到峰值；Ｃｈｉｔｏｓａｎ浓度在１００ｍｇ·Ｌ－１时对红豆杉细胞的生长基本无抑制作用，增产效果最显著（约为对照组的１０倍），Ｃｈｉｔｏｓａｎ可作为Ｔａｘｏｌ合成的诱导子．The effects of chitosan on the improvement of taxol (an anticancer drug) biosynthesis and phenylalanine ammonia lyase (PAL) activity in Taxus chinensis suspension cultures were studied. T. Chinensis cells were cultured in modified MS liquid medium, and chitosan were incorporated to the medium at different concentrations on 18th day during the culturing period. PAL activity were transient elicited and peaked at 16h after chitosan were added into T. Chinensis cell cultures at a terminal concentration of 100￣1000mg·L -1 . PAL activity increased with chitosan concentrations. Chitosan could increase taxol yield, especially at 100mg·L -1 . Chitosan not only did no harm to the cell growth of yew suspension cultures but had the highest taxol yield improvement (10 fold vs.control). Chitosan should be a novel elicitor of taxol biosynthesis.

关 键 词：红豆杉 聚氨基葡糖 PAL 紫杉醇 合成 细胞培养 

分 类 号：R284.3[医药卫生—中药学] S791.49[医药卫生—中医学]