外源水杨酸诱导水稻相关防御酶活性及内源水杨酸含量的变化  

Changes of Resistance-Related Defense Enzymes Activities and Endogenous Salicylic Acid in Rice Induced by Exogenous Salicylic Acid

作　　者：张智慧[1] 聂燕芳[2] 何磊[1] 李云锋[1] 王振中[1] 

机构地区：[1]华南农业大学植物病理生理学研究室/教育部生物防治工程研究中心,广州510642 [2]华南农业大学制药工程系,广州510642

出　　处：《华中农业大学学报》2010年第5期541-545,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30971887;30600399);广东省自然科学基金项目(07006683;05300351)资助

摘　　要：采用0.1mmol/L的水杨酸(salicylicacid,SA)喷雾处理抗稻瘟病近等基因系水稻CO39和C101LAC。结果表明:脂氧合酶(LOX)、苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)活性在不同亲和性互作水稻中均在早期上升,与亲和性互作水稻CO39相比,高度非亲和性互作水稻C101LAC3种酶的诱导活性增加明显、速度快;过氧化氢酶(CAT)的诱导活性则在不同亲和性互作水稻中均下降。对病程相关蛋白(PR蛋白)β-1,3-葡聚糖酶和几丁质酶的测定结果表明,高度非亲和性互作水稻2种酶诱导活性的出现高峰期和强度也明显要早且高于亲和性互作水稻。对内源水杨酸的测定结果表明,不同亲和性互作水稻的SA含量均没有明显的变化,表明外源SA诱导的水稻抗病性可能与内源SA信号通路无关。Salicylic acid（SA）is an important signaling hormone in plants,playing a role in induced defense responses.After treatment with exogenous SA,changes in liposygenase（LOX）activity,phenylalanine ammonialyase（PAL）activity and peroxidase（POD）activity increased significantly compared with those in contro1.The activity of three enzymes increased more remarkably and rapidly on the leaves of the incompatible rice line than those in the compatible rice line.CAT activity decreased on the leaves of two rice cultivars.Pathogenesis-related proteins（PR proteins）are a characteristic of systemic acquired resistance（SAR）in plants.The results showed that changes in two PR proteins,chitinase and β-1,3-glucanase,were also increased more remarkably and rapidly on the leaves of the incompatible rice line than on those of the compatible rice line.The results also showed that endogenous SA had little change either on the leaves of the two rice cultivars after inoculation with exogenous SA.The results suggest that defense-related enzymes and SAR in rice leaves induced by exogenous SA might play an important role in rice resistance to rice blast.

关 键 词：水杨酸 水稻 防御酶 PR蛋白 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]