莲叶海棠组织培养及快速无性繁殖  

Study on the Rapid Clonal Propagation of Begonia feastii

作　　者：韩阳[1] 吴丽馥[1] 侯潇 娄和林[1] 赵彦芳[1] 

机构地区：[1]辽宁大学生物系

出　　处：《辽宁大学学报（自然科学版）》1999年第2期179-181,共3页Journal of Liaoning University：Natural Sciences Edition

摘　　要：以莲叶海棠幼叶为材料，材料经表面消毒接种于诱导培养基上，２７ｄ左右外植体表面出现不定芽．经继代培养可获大量不定芽．不定芽在壮苗培养基上培养４５ｄ转至生根培养基中，４０ｄ后长出完整根系．再生植株炼苗后转移至砂基中进行培养，移栽成活率为９０％．The young leaves of Begonia feastii were used as explant material and cultured on the inducing media. The results show that a number of adventitous shoots grew on the surface of explant after 27 days. Many adventitious shoots were acquired through proliferating culture. After they has grown on nutritious media for 45 days, they were transferred on the inducing root media. After 40 days, every shoot outgrew a number of roots and developed into a whole plantlet. The regeneration plantlet were replanted in sand, about 90 percent of the plantlets survived.

关 键 词：再生植株 快速繁殖 莲叶海棠 无性繁殖 组织培养 

分 类 号：S685.120.3[农业科学—观赏园艺]