武植3号中华猕猴桃微卫星DNA变异的研究  

Study on Microsatellite DNA Variance in Actinidia chinensis cv.Wuzhi No.3

作　　者：潘伟明[1] 刘文[2] 杨妙贤[2] 刘胜洪[2] 梁红[2] 

机构地区：[1]仲恺农业工程学院科技处,广州510225 [2]仲恺农业工程学院生命科学学院,广州510225

出　　处：《湖北农业科学》2010年第10期2344-2347,2352,共5页Hubei Agricultural Sciences

基　　金：广东省自然科学基金项目(8151022501000010)

摘　　要：利用9对引物对湖北省建始县、广东省和平县两地的中华猕猴桃品种武植3号(Actinidia chinen-sis Planch.cv Wuzhi No.3)栽培群体及单株不同叶片进行了SSR(Simple sequence repeats)分析。结果表明,武植3号的微卫星DNA在长期的无性繁殖和栽培过程中产生了随机变异,主要表现为某些微卫星位点的缺失和一些位点的增加。通过统计表明,广东省和平县栽培的武植3号微卫星位点总变异的频率较高,达0.1747,湖北省建始县的为0.0461,两者差异明显。研究认为,长期栽培和连续多次嫁接扩繁是武植3号SSR变异增加的主要因素。SSR（Simple sequence repeats）variances of Actinidia chinensis Planch.cv.Wuzhi No.3 cultivated in Heping county of Guangdong province and Jianshi county of Hubei province were investigated by application of 9 pairs of primers in PCR amplification.The results showed that random variation of microsatellite DNA occurred in the vegetative reproduction populations with loss or increase of some SSR loci.Total variation frequency of A.chinensis cv.Wuzhi No.3 from Heping county was 0.174 7 and that from Jianshi county was 0.046 1,which suggested that the higher SSR variation of the kiwifruit population grown in Heping county mainly resulted from repeated vegetative reproduction during long cultivation period.

关 键 词：中华猕猴桃 微卫星DNA 简单重复序列变异 

分 类 号：S663.4[农业科学—果树学] Q78[农业科学—园艺学]