草莓炭疽病病原菌的快速鉴定  

Rapid Identification of Colletotrichum Spieces from Strawberry

作　　者：吴金平[1] 曾祥国[1] 宋志红[1] 向发云[1] 吴娟[2] 冯小明[1] 顾玉成[1] 

机构地区：[1]湖北省农业科学院经济作物研究所,武汉430064 [2]安庆师范学院生命科学学院,安徽安庆246011

出　　处：《湖北农业科学》2010年第10期2437-2438,2448,共3页Hubei Agricultural Sciences

基　　金：国家科技支撑计划资助项目(2008BAD92B04-06)

摘　　要：草莓炭疽病近年来越来越严重,尤其在高温育苗阶段。为了减少该病害的危害,搞清楚其致病菌就显得尤为重要。试验利用PCR扩增真菌保守的ITS序列,确定其分类地位,避免了传统分类方法的繁琐、耗时和结果的不稳定性。结果表明,所收集的7个菌株中有6株为胶孢炭疽菌(Colletotrichum gloeosporioides),仅1株为尖孢炭疽菌(Colletotrichum acutatum)。Strawberry anthracnose is more and more serious in recent years, especially in the high-temperature propagation stage. In order to reduce threaten of this disease, it is important to understand the characteristic of the pathogen. With the rapid development of molecular biology, the taxonomic status was determined by PCR techniques which amplify the fungal ITS conservative sequences, avoiding tedious, time-consuming and unstable the traditional classification. The results showed that six strains were attributed to Colletotrichum gloeosporioides, only one strain was classified as Colletotrichum acutatum.

关 键 词：草莓炭疽病 鉴定 核糖体基因转录间隔区 

分 类 号：S436.639[农业科学—农业昆虫与害虫防治]