MK-801对锰致神经元损伤的保护作用  

PROTECTIVE EFFECTS OF MK-801 ON MANGANESE-INDUCED NEURONAL DAMAGE

作　　者：徐斌[1] 徐兆发[1] 邓宇[1] 黄晓曦[1] 

机构地区：[1]中国医科大学公共卫生学院环境卫生学教研室,沈阳110001

出　　处：《现代预防医学》2010年第20期3888-3890,共3页Modern Preventive Medicine

基　　金：国家自然科学基金(30771834)

摘　　要：[目的]研究不同浓度锰对原代培养神经元的损伤情况,同时用MK-801预处理,观察其对锰致神经元损伤的保护作用。[方法]选用原代培养神经元为模型,待细胞生长至最佳状态时,予以分组处理。染锰组为含不同浓度氯化锰25、100、400μmol/L的培养液,MK-801预处理组,用10μmol/LMK-801预处理30min后,再用400μmol/L氯化锰处理神经元,对照组为正常培养液,通过检测细胞活力、乳酸脱氢酶(LDH)释放量以及用TUNEL技术检测细胞凋亡的方法,评价MK-801对锰致神经元损伤的保护作用。[结果]用不同剂量锰处理神经元6～48h发现,神经元胞体固缩,突起断裂,网络消失。细胞活力和LDH释放量在同一染毒时间,随锰浓度升高细胞活力逐渐降低,LDH释放量逐渐升高;在同一锰浓度处理神经元,随着时间的延长细胞活力逐渐降低,LDH释放量逐渐升高。随锰浓度的升高,神经元细胞凋亡率和积分光密度均逐渐上升。用MK-801预处理30min后,400μmol/L锰处理神经元发现,与单纯用400μmol/L锰处理比较,细胞活力回升,LDH释放量降低,细胞凋亡率和积分光密度均有所下降。[结论]锰可以剂量依赖性和时间依赖性的对神经元造成损伤,促使细胞凋亡;MK-801可以在一定程度上有效地保护神经元。[Objective] To study different concentration manganese（Mn） induced cell damage in primary cultured neurons.After neurons pre-treated with MK-801,it was observed the protective effects of MK-801 on manganese-induced neuronal damage.[Methods] The primary cultured neurons provided an ideal in vitro model system.When growth status of neuron was good,we perform the following experiment.Primary neuronal cells were treated with fresh media with different concentrations of Mn（0,25,100,400 μM） prewashed with media and pretreated with MK-801 at a concentration of 10 μM for 30 min prior to 400μM Mn.By MTT,lactate dehydrogenase（LDH） and TUNEL assay,we evaluated the protective effects of MK-801 on manganese-induced neuronal damage.[Results] Neuronal cells was treated with different concentration Mn for 6-48 h resulted in the cell bodies shrinkage,the neuraxon of neurons shortened,and the network disappeared.With the increase of Mn concentration and prolong of Mn treatment period,the viability of neurons decreased and levels of LDH increased.Mn therapy could result in a significant increase of apoptotic cell death in a concentration-dependent manner.Pretreatment with MK-801 at a concentration of 10 μM for 30 min prior to 400 μM Mn prevented the alterations of the activities of the viability of neurons,LDH release and apoptotic cell death.[Conclusion] Treatment of neuronal cells with Mn could induce the damages of primary cultured neurons concentration-and time-dependently.MK-801 may effectively protect Mn-induced cell damage in certain extent.

关 键 词：锰 MK-801 原代培养神经元 细胞凋亡 

分 类 号：R971[医药卫生—药品]