定量与半定量酶联免疫吸附试验在麻疹IgG抗体检测中的比较  

COMPARATIVE STUDY ON APPLICATION OF QUANTITATIVE ELISA AND SEMI-QUANTITATIVE ELISA FOR DETECTION OF MEASLES ANTIBODY

作　　者：张腾[1] 郑维斌[2] 李园园[2] 李立群[3] 何丽芳[3] 李洪[1,3] 陆林[1,3] 

机构地区：[1]云南昆明医学院公共卫生学院,昆明650031 [2]保山市疾病预防控制中心 [3]云南省疾病预防控制中心

出　　处：《现代预防医学》2010年第20期3938-3939,3947,共3页Modern Preventive Medicine

摘　　要：[目的]通过对麻疹病毒IgG抗体定量酶联免疫吸附试验(ELISA)与传统半定量酶联免疫吸附试验(ELISA)进行比较,评价传统半定量ELISA法的实用价值。[方法]随机抽取2008年的180份云南省保山市两所边远小学小学生血清,用德国维润公司定量ELISA试剂盒测定麻疹IgG抗体。这180血清已经用半定量方法将抗体滴度测出,之后将两种方法测定的结果进行比较。[结果]定性结果分析发现,180份血清标本中,8份阴性,3份可疑,162份阳性,与传统半定量ELISA方法的一致率为96.05%,统计分析两实验结果差异无统计学意义(P﹥0.05),Kappa检验得两法存在高度一致性(Kappa值=0.677,P﹤0.001)。同时得出传统半定量法的灵敏度为95.86%,特异度为100%,阴性似然比为0.04,正确指数为95.86%,阳性预测值为98.18%,阴性预测值为53.33%。定量测定方面,随着抗体滴度的增高,该组血清抗体国际单位(IU)也随之上升,抗体滴度分组与抗体IU明显的正相关,相关系数(rs)为0.41,P﹤0.001。[结论]通过本次定量与半定量ELISA法在麻疹IgG抗体检测中的比较发现,半定量法的定性、定量结果一定程度上已经能够较好反映人群麻疹抗体水平,对麻疹的监测仍具有重要的实用意义。[Objective] To compare the quantitative ELISA with traditional semi-quantitative ELISA for detection of measles IgG antibody.[Methods] 180 samples of serum from two primary schools of Baoshan county were tested by German Vrion-Serion regent,which had been tested by semi-quantitative method.[Results] By qualitative analysis,among 180 samples,8 samples were negative,3 samples were suspicious,162 samples were positive,and the consistency rate was 96.05% with traditional semi-quantitative ELISA,there was no significant difference between the two methods（P﹥0.05）.The consistency showed to be high by Kappa test.The sensitivity of semi-quantitative method was 95.86%,and the specificity of semiquantitative method was 100%;the Youden,s index was 95.86%,the negative likelihood ratio was 0.04;the positive predictive value was 98.18%,and the negative predictive value was 53.33%.Quantitative analysis showed that the test value and meadian of international unit of serum antibody increased with the dilution ratio increased.Dilution ratio and international unit were positively relative,and the relative coefficient rs was 0.41（P﹤0.001）.[Conclusion] The semi-quantitative and quantitative results indicated that the quantitative and qualitative results of IgG antibody level could reflect the level of measles in population,and the quantitative and qualitative results of semi-quantitative results has significant effect in the surveillance of measles.

关 键 词：麻疹 IGG抗体 定量酶联免疫吸附试验 半定量酶联免疫吸附试验 

分 类 号：R511.1[医药卫生—内科学]