宣木瓜中多糖含量测定  

Determination of Polysaccharide Content in Chaenomeles speciosa(Sweet) Nakai

作　　者：李娜[1,2] 张卫明[1,2] 姜洪芳[2] 金敬宏[2] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《中国野生植物资源》2010年第5期55-57,共3页Chinese Wild Plant Resources

摘　　要：目的：建立宣木瓜中多糖含量的测定方法,并测定不同采收时期的宣木瓜多糖含量。方法：在同一种植基地定期采集同一宣木瓜品种果实,以葡萄糖为对照品,采用分光光度法,以苯酚—浓硫酸为显色剂,在490 nm波长处测定吸光度,计算样品中多糖含量。结果：宣木瓜果实在生长过程中,其多糖含量在2.51%~5.08%之间,并于7月8日含量最高为5.08%。结论：本文建立的宣木瓜多糖含量的测定方法简便灵敏,精密度高,重现性好,稳定可靠;若要利用多糖含量高的宣木瓜,应适宜于7月上旬或中旬采收。Objective：A method for determination the content of polysaccharide in Chaenomeles speciosa（Sweet） Nakai was established,and it determined the content of polysaccharide in Chaenomeles speciosa（Sweet） Nakai by different harvest times.Method： Samples were picked regularly in the same base,and polysaccharide content of samples was tested by spectrophotometry using glucose as the standard sample,color was developed with phenol-sulfuric acid and absorbance was read at 490 nm.Result： The polysaccharide content was 2.51%-5.08% during the fruit growth process,and the highest content was on July 8.Conclusion： The method for determination the content of polysaccharide in Chaenomeles speciosa（Sweet） Nakai was simple,sensitive,high precision,reproducible,stable and reliable;To take advantage of Chaenomeles speciosa（Sweet） Nakai with high polysaccharide content,the best harvest period should be early or mid-July.

关 键 词：宣木瓜 多糖 分光光度法 采收期 含量测定 

分 类 号：R284.1[医药卫生—中药学]