蝗虫染色体分带技术的研究  

A Study of Locust's Chromosomal Banding Technology

作　　者：马恩波[1] 姚爱玉[1] 

机构地区：[1]山西大学生命科学系

出　　处：《遗传》1999年第3期28-30,共3页Hereditas（Beijing)

基　　金：国家自然科学基金

摘　　要：本文对采自长沙岳麓山的中华稻蝗〔Ｏｘｙａｃｈｉｎｅｎｓｉｓ（Ｔｈｕｎｂｅｒｇ）〕和采自杭州的小稻蝗〔Ｏｘｙａｈｙｌａｉｎｔｒｉｃａｔａ（Ｓｔａｌ）〕进行了染色体Ｃ带、Ｒ带、Ｎ带和Ａ带的处理研究。结果表明，Ｃ带显示结构异染色质，其位置在不同分裂相中较为稳定；Ｒ带即Ｇ带的反带，带纹较Ｃ带丰富，在粗线期最为明显，本文观察到不同的温育条件可影响Ｒ带产生的效果；Ｎ带和Ａｇ带可相互参照比较，从而为ＮＯＲ的准确定位提供依据；Ａ带即荧光带，本文对其显带程序作了一定的改进。In this paper Oxya chinensis (Thunberg) collected from the Yuelu Mountains in Changsha, Hunan Province and Oxya hyla intricata (Stal) collected from Hangzhou, Zhejiang province were analysed by chromosomal C-banding、R-banding、N-banding、Ag-banding and A-banding. The results showed that the constitutive heterochromatin was stained in C-banding, and it relatively stable; R-banding was the reverse of G-banding, its bands were relatively obvious at the pachytene and abundent, it may be because of the selective extracts that cause the bands evident, the different effects were surveyed under the various incubation conditions; Both N-banding and Ag-banding can stain the nucleolar organizer and the two were compared in this paper; The A-banding was a little improved in the light of the banding process.

关 键 词：蝗虫 染色体 分带技术 

分 类 号：Q969.265.2[生物学—昆虫学] Q962