反硝化细菌的筛选及其亚硝酸盐降解特性研究  

Isolation and nitrite degradation ability of a denitrifying bacterium

作　　者：罗秀针[1] 徐长安[2] 唐旭[2] 何建林[2] 

机构地区：[1]漳州卫生职业学院,福建漳州363005 [2]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《福建农业学报》2010年第4期513-516,共4页Fujian Journal of Agricultural Sciences

基　　金：厦门海洋研究开发院资助项目(K09102-1)

摘　　要：从海水养殖池的底泥中筛选到6株反硝化细菌,其中对亚硝酸盐降解力最强的1株菌JN1初步鉴定为假单胞菌,研究了JN1的亚硝酸盐降解特性,结果表明：JN1具有高效降解转化亚硝酸钠的能力,其适宜降解条件为：亚硝酸钠浓度1 000~4 000 mg.L-1,菌种接种量5%（v/v）,培养基装量100 mL（250 mL容量）,中量充气发酵,发酵时间18~30 h,发酵pH值6.5~7.5。Six strains of denitrifying bacteria were isolated from the mud in marine aquaculture ponds.Among them,one showed the strongest nitrite degradation capability.The strain was subsequently identified to be a Pseudomonas sp.and named JN1.Its optimum nitrite degradation conditions were determined in the lab to be at 1 000-4 000 mg·L-1 sodium nitrite concentration,5%（v/v） bacterial inoculums and pH 6.5-7.5 with 100 mL of fill volume in a 250 mL flask and moderate aeration in the fermentation for 18-30 h.

关 键 词：反硝化细菌 亚硝酸盐 降解 

分 类 号：Q939.9[生物学—微生物学]