麻鸡脾脏总RNA提取方法初探  

Study on the Extraction of Total RNA in Ma Chicken's Spleen

作　　者：陈书明[1] 常云花[1] 梁新峰[1] 曹新鹏[1] 范瑾[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《山西农业科学》2010年第10期51-52,64,共3页Journal of Shanxi Agricultural Sciences

基　　金：山西省科技攻关项目(052011);山西高校科技研究开发项目(20051222);山西农业大学科技创新基金项目(200002)

摘　　要：为了从麻鸡脾脏组织中提取出纯度高、完整性好的总RNA,将约80,120,140 mg的麻鸡脾脏组织分别与1 mL的RNAisoPlus混合,采用RNAisoPlus法进行提取。从提取出的RNA琼脂糖凝胶电泳图谱可知,当脾脏组织用量约140 mg时,RNA几乎全部降解;当脾脏组织用量约120 mg时,RNA降解严重;当脾脏组织用量控制在约80 mg时,提取出的RNA电泳图谱为清晰的3条带,分别为5 S,18 S,28 S,且A260/A280=1.982。上述结果表明,用1 mL的RNAiso Plus,并且把脾脏组织用量控制在80 mg左右时,可提取出纯度高、完整性好的总RNA。In orderto extract total RNA of high purity and integrity from Ma chicken’s spleen,about 80,120,140 mg tissue of the chicken’s spleen were mixed with 1mLRNAiso Plus reagent,using RNAiso Plus,the total RNA fromthe chicken’s spleen tissue were extracted.It was shown on the agarose gel electrophoresis mapping of the extracted RNA： when about 120 mg spleen tissue was used,the RNA degraded seriously;when about 140 mg spleen tissue was used,the RNA almost degraded;and when about 80 mg spleen tissue was used,three strips of the agarose gel electrophoresis mapping were showed as 5 S,18 S,28 S respec-tively and A260/A280=1.982.The results suggested that,when 1mL RNAiso Plus reagent and about 80 mg spleen tissue were mixed,the total RNA of high purity and integrity could be extracted from the chicken’s spleen.This study provides the way for future study of cloning gene concerned by RT-PCR.

关 键 词：麻鸡 脾脏 总RNA RNAisoPlus 

分 类 号：Q781[生物学—分子生物学]