甘氨酸对鲫鱼视网膜ERG b-波和ON型双极细胞活动的调制  

GLYCINE MODULATES THE ERG bWAVES AND ONTYPE BIPOLAR CELLS IN CARP RETINA*

作　　者：徐祥敏[1,2] 杨雄里[1,2] 

机构地区：[1]中国科学院上海生理研究所 [2]中国科学院神经生物学开放实验室

出　　处：《生理学报》1999年第2期121-127,共7页Acta Physiologica Sinica

基　　金：国家科委"攀登计划";上海生命科学中心资助

摘　　要：本工作在分离灌流的鲫鱼视网膜上研究了甘氨酸对明、暗视视网膜电图（ＥＲＧ）ｂ波和胞内记录的ＯＮ型双极细胞反应的作用。结果表明，甘氨酸能明显压抑ＥＲＧｂ波和ＯＮ型双极细胞的反应，其作用能为士的宁所翻转；甘氨酸对用谷氨酸分离的ＥＲＧＰⅢ成分（光感受器电位）无明显影响。这些结果提示，甘氨酸可能直接作用于双极细胞的受体，从而调节视网膜ＯＮ通路的活动。In the present work we investigated the effects of glycine on the electroretinograms (ERG) and light responses of ONtype bipolar cells under dark and lightadapted conditions in superfused, isolated crucian carp retinas. It was revealed that application of 4 mmol/L glycine significantly suppressed the bwaves and the responses of ONtype bipolar cells and the effects were blocked by coapplication of 20 mol/L strychnine. In addition, glycine had no apparent action on the P component (photoreceptor potential) isolated by application of 3 mmol/L glutamate. These results suggest that glycine may act on bipolar cells directly and thus modulate the activity of the ON pathway in the retina.

关 键 词：基氨酸 视网膜电图 ON型 双极细胞 调制 

分 类 号：Q436[生物学—生理学]