皮质醇对离体牛蛙交感神经节B细胞胆碱能突触传递的快速作用  

RAPID EFFECTS OF HYDROCORTISONE ON THE CHOLINERGIC SYNAPTIC TRANSMISSION OF B NEURONS IN BULLFROG SYMPATHETIC GANGLIA

作　　者：易必达[1,2] 马蓓[1,2] 邢宝仁[1,2] 

机构地区：[1]第二军医大学生理教研室 [2]贵阳医学院生理教研室

出　　处：《生理学报》1999年第2期147-152,共6页Acta Physiologica Sinica

摘　　要：在离体灌流的牛蛙交感神经节标本上，电刺激节前纤维，细胞内记录Ｂ细胞的电活动，观察给予皮质醇对Ｂ细胞突触传递的快速作用。主要结果有：（１）电刺激节前纤维，细胞内记录１７０个Ｂ细胞的动作电位，给予皮质醇后０５～３ｍｉｎ内，５２个Ｂ细胞的突触传递发生脱漏甚至完全阻断，有明显的量效关系。甾体激素胞内受体阻断剂ＲＵ３８４８６可部分阻断这种作用。（２）蛋白合成抑制剂放线菌酮不能阻断皮质醇的快速阻断作用。（３）阿托品阻断Ｍ受体后，皮质醇对Ｂ细胞突触传递的快速阻断作用增强。上述结果提示，皮质醇通过非基因组机制对牛蛙交感神经节Ｂ细胞的突触传递产生快速阻断作用。Rapid effects of hydrocortisone 21hemisuccinate (Fsuc) on cholinergic synaptic transmission of B neurons in bullfrog sympathetic ganglia (BFSG) were studied with intracellular recording technique in vitro. The main results are as follows: (1)Fsuc blocked cholinergic synaptic transmission of 52 B neurons within 053 min, which was concentrationdependent and partially suppressed by the cytoplasm receptor antagonist of steroid hormones, RU38486; (2)after protein synthesis was suppressed by actidione, the above effect persisted; and (3)the effect of Fsuc could be potentiated by atropine. All these results suggest that the Fsuc induced rapid effects of cholinergic synaptic transmission of B neurons in BFSG may be mediated by nongenomic mechanisms.

关 键 词：皮质醇 交感神经节 快速作用 突触传递 

分 类 号：Q577[生物学—生物化学] R338.13[医药卫生—人体生理学]