一氧化氮/内皮素在大鼠胃粘膜损伤中的作用及电针的调整效应  

THE ROLE OF NO/ET AND THE EFFECT OF ELECTROACUPUNCTURE ON INJURIED GASTRIC MUCOSA IN RATS

作　　者：孙勇[1] 许冠荪[1] 刘维平[1] 许能贵[1] 

机构地区：[1]安徽中医学院针灸经络研究所生理研究室

出　　处：《生理学报》1999年第2期206-210,共5页Acta Physiologica Sinica

基　　金：国家攀登计划

摘　　要：用酒精灌胃引起大鼠胃粘膜损伤模型，观察内皮衍生因子（ＮＯ／ＥＴ）的含量变化和电针对胃粘膜损伤调整作用。结果发现：酒精灌胃后，胃粘膜血流量（ＧＭＢＦ）、跨壁电位差、血ＮＯ含量降低（Ｐ＜００１），血浆ＥＴ含量和胃粘膜损伤指数（ＬＩ）增高（Ｐ＜００１）。Ｌ精氨酸（ＬＡｒｇ）或硝普钠（ＳＮＰ）灌注预处理后（ｉｖ），ＮＯ含量和ＧＭＢＦ明显升高（Ｐ＜００１），ＥＴ含量和ＬＩ指数下降（Ｐ＜００１）。电针（ＥＡ）足三里穴位加强了以上效应，且ＥＡ效应被预先静脉灌注的ＮＯ生物合成抑制剂Ｎ亚硝基左旋精氨酸（ＬＮＮＡ）所抑制。ＬＮＮＡ的抑制效应又可被与其同时灌注的ＬＡｒｇ所翻转。结果提示：ＮＯ有抗胃粘膜损伤作用，电针对胃粘膜保护作用的主要效应分子可能是ＮＯ。The aim of the present work is to observe changes in endotheliumderived factors nitric oxide(NO)and endothelin(ET), with special reference to the regu.lative role of electroacupuncture(EA)in injured gastric mucosa of rat by ethanol. It was found that gastric mucosal blood flow(GMBF), transmucosal potential difference(PD)and the content of NO in the serum all decreased (P<001), while the content of ET in the plasma and the lesion index(LI) of gastric mucosa increased(P<001). After administration of LArg and SNP, the content of NO and GMBF increased significantly(P<001), whereas the content of ET and LI decreased (P<001). These effects were enhanced by EA at ZuSanLi (ST36) points, which, however, were inhibited by NO biosynthetic inhibitor LNNA. The latter effect could be reversed by concurrent administration of LArg. The above results suggest that NO plays a critical part in protecting gastric mucosa against injury, which is also responsible for the protective effect of EA on injury of gastr

关 键 词：胃粘膜损伤 一氧化氮 内皮素 电针 

分 类 号：R245.97[医药卫生—针灸推拿学] R259.73[医药卫生—中医临床基础]