bFGF增强大鼠低氧/复氧损伤心脏成纤维细胞β-catenin表达  

bFGF increases expression of β-catenin in the damaged cardiac fibroblasts of rats with hypoxia/reoxygenation

作　　者：牛欢[1] 黄晏[1] 张胜叶[1] 赵乾[1] 

机构地区：[1]兰州大学第一医院心血管内科,甘肃兰州730000

出　　处：《基础医学与临床》2010年第10期1085-1088,共4页Basic and Clinical Medicine

摘　　要：目的探讨碱性成纤维细胞生长因子(bFGF)对心脏成纤维细胞低氧/复氧损伤后β-catenin蛋白表达的影响。方法制作体外培养第3代大鼠心脏成纤维细胞低氧/复氧(Hy/R)损伤模型,分为6组:对照组、单纯低氧/复氧损伤(Hy/R)组、以及5、10、20、和40μg/L bFGF干预组,采用MTT法、TUNEL法、免疫荧光法及激光共聚焦显微镜分析检测心脏成纤维细胞增殖率、凋亡百分率和β-catenin蛋白表达。结果单纯Hy/R组较对照组细胞增殖率降低(P<0.05),β-catenin蛋白表达减少(P<0.05),凋亡细胞增多(P<0.05)。20和40μg/L bFGF干预组较单纯Hy/R组细胞增殖率升高(P<0.05),β-catenin蛋白增多(P<0.05),凋亡细胞减少(P<0.05)。20、40μg/LbFGF干预组较对照组细胞增殖率、β-catenin蛋白表达、凋亡百分率均有显著改变(P<0.05)。结论bFGF提高Wnt通路中β-catenin蛋白的表达,促进心脏成纤维细胞增殖并减少Hy/R损伤后心脏成纤维细胞凋亡。Objective To explore the regulative effect and mechanism of basic fibroblast growth factor（bFGF） to β-catenin in cardiac fibroblasts.Methods SD rat cardiac fibroblasts（CFs） were isolated and cultured in medium and then in hypoxia/reoxygenation（Hy/R） condition.We used the third generation cardiac fibroblasts and divided into 6 groups,including the control group,the Hy/R control group,the 5,10,20 and 40 μg/L bFGF group.The MTT method was used to investigate the proliferation of cardiac fibroblasts,The expression of β-catenin and apoptosis cell were examined and measured by laser confocal scanning microscope method and TUNEL method.ResultsMTT A490 nm value in Hy/R groups was significant decreased compared with control group（P0.05）,with β-catenin protein decreased（P0.05） and apoptosis increased（P0.05）.Compared with Hy/R groups the MTT A490 nm value of the bFGF groups was significantly increased（P0.05）,the expression of β-catenin in bFGF group was increased significantly（P0.05）,and the cell apoptosis was decreased in bFGF group（P0.05）.Conclusion bFGF can up-regulate the expression of β-catenin in the damaged cardiac fibroblast,facilitates cell proliferation and depresses cell apoptosis after Hy/R.

关 键 词：低氧/复氧损伤 碱性成纤维细胞生长因子 β联接蛋白 凋亡 

分 类 号：R542.22[医药卫生—心血管疾病]