胡杨中两个新DREB类基因的克隆、序列分析及转录激活功能研究  

Isolation,sequence analysis and transcriptional activation study of two novel DREB genes from Populus euphratica Oliv

作　　者：陈金焕[1] 叶楚玉[1] 夏新莉[1] 尹伟伦[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室,林木花卉遗传育种教育部重点实验室

出　　处：《北京林业大学学报》2010年第5期27-33,共7页Journal of Beijing Forestry University

基　　金："十一五"国家科技支撑计划项目(2006BAD03A01);国家自然科学基金项目(30730077;30972339);"863"国家高技术研究发展计划项目(2007AA10Z106);优秀青年教师科技支撑专项计划项目(YX2010-17)

摘　　要：DREB转录因子(干旱应答元件结合因子)是逆境适应中的关键调节因子。该类转录因子的特点是拥有保守的AP2/EREBP(APETALA2/乙烯应答元件结合蛋白)结构域,能够特异性地与抗逆基因启动子上的DRE元件相结合,在低温、干旱和盐碱等条件下调节一系列下游逆境应答基因的表达。本研究从胡杨中分离出2个编码DREB2类蛋白的基因PeDREB3和PeDREB4。PeDREB3和PeDREB4在胡杨根、茎、叶中都有表达。序列分析显示,PeDREB3和PeDREB4均含有非典型性AP2/ERF结构域,即在AP2/EREBP结构域的第2个β折叠和第3个β折叠处多出8个氨基酸残基,用改进的酵母单杂交技术发现PeDREB3和PeDREB4能够在酵母中激活下游报告基因的表达,具有转录活性。Dehydration-responsive element binding（DREB） transcription factors with a typical AP2 /EREBP DNA-binding domain might recognize and bind to DRE cis-element,especially and finally activate a lot of stress-responsive genes when plants are under abiobitc stresses,including dehydration,high salt and low temperature.Using PCR and homologous DREB search,we isolated two DREB2-type genes from poplar（Populus euphratica Oliv.） named PeDREB3 and PeDREB4.PeDREB3 and PeDREB4 distinguished themselves from other DREBs with an 8 bp extension sequence between their second and third β-sheets of the AP2 domain.Modified yeast one hybrid analysis reveals that full length conding sequence has transcriptional activation in yeast.

关 键 词：胡杨 DREB转录因子 AP2/EREBP结构域 转录活性 

分 类 号：Q78[生物学—分子生物学]