溴氰菊酯对不同品系家蝇脑突触体膜蛋白磷酸化及ATP酶活性的影响  

INHIBITION OF PROTEIN PHOSPHORYLATION AND ATPase ACTIVITY BY DELTAMETHRIN IN HOUSEFLY BRAIN SYNAPTOSOMES

作　　者：田雨[1] 冷欣夫[1] 

机构地区：[1]中国科学院动物研究所

出　　处：《昆虫学报》1999年第2期113-119,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金

摘　　要：以敏感品系家蝇和溴氰菊酯抗性品系家蝇（ＭｕｓｃａｄｏｍｅｓｔｉｃａＬ．）为材料，研究和比较了神经毒剂溴氰菊酯对其脑突触体蛋白磷酸化作用的影响。结果表明，浓度为１０－５ｍｏｌ／Ｌ溴氰菊酯抑制了敏感品系家蝇脑突触体蛋白磷酸化作用，而对抗性品系家蝇脑突触体蛋白磷酸化作用无明显影响。若反应体系中加入２５×１０－６ｍｏｌ／Ｌ的ｃＡＭＰ显著激活了敏感品系家蝇脑突触体蛋白磷酸化水平，但是当０６ｍｍｏｌ／ＬＣａ２＋或０６ｍｍｏｌ／ＬＣａ２＋加１０－５ｍｏｌ／Ｌ钙调蛋白时明显增强了抗性品系家蝇脑突触体蛋白磷酸化水平，甚至超过了其对敏感品系的作用水平。此外，还发现不同浓度的溴氰菊酯可抑制突触膜上的Ｎａ／ＫＡＴＰ酶和ＣａＡＴＰ酶活力，浓度越高抑制作用也越大。Deltamethrin was found to affect ATPase activity and protein phosphorylation of housefly brain synaptosome in vitro . Both Na,K ATPase and Ca ATPase activities can be inhibited by various concentrations of deltamethrin (10 -9 ～10 -4 mol/L). However, the inhibition was higher in susceptible housefly than in deltamethrin resistant strain. In intact susceptible housefly brain synaptosome the most significant result was inhibition of endogenous protein phosphorylation by 10 -5 mol/L of deltamethrin. Such effect in deltamethrin resistant housefly was suppressed by adding the exogenous Ca 2+ or Ca 2+ and calmodolin to the reaction system.Cyclic AMP stimulated the overall phosphorylation level,particularly 48 kD protein in susceptible housefly. The 48 kD protein,isoelectric point pI 5 2,may be the brain specific B 50 protein in housefly.All these results indicated that:(1)Deltamethrin has the property to affect ATPase activity and protein phosphorylation.(2)Deltamethrin might limits calcium transport which connects with the protein phosphorylation.

关 键 词：溴氰菊酯 家蝇 脑突触膜 蛋白磷酸化 ATP酶 

分 类 号：Q965.9[生物学—昆虫学] S482.3[农业科学—农药学]