EPC-K1防止ONOO^-诱导的小脑颗粒神经元凋亡  

EPC K1 Prevents ONOO - induced Apoptosis in Cerebellar Granule Neurons

作　　者：卫涛涛[1] 陈畅[1] 黄勇进 赵保路[1] 侯京武[1] 忻文娟[1] MORI Akitane 

机构地区：[1]中国科学院生物物理研究所

出　　处：《生物化学与生物物理学报》1999年第3期239-244,共6页

基　　金：中国科学院九五重点项目

摘　　要：荧光显微镜观察及ＥＬＩＳＡ分析表明１０μｍｏｌ／Ｌ过氧化亚硝基可诱导原代培养的大鼠小脑颗粒神经元凋亡。应用ＥＳＲ自旋标记技术研究了这一过程中细胞膜生物物理特性的变化，结果表明神经细胞经过氧化亚硝基处理后，细胞膜表层及深层流动性均显著下降，膜蛋白巯基强弱固定化比值增加。维生素Ｃ、维生素Ｅ的衍生物Ｌ抗坏血酸２［３，４二氢２，５，７，８四甲基２（４，８，１２三甲基十三烷基）２Ｈ１苯并吡喃６磷酸酯］钾盐（ＥＰＣＫ１）能减轻过氧化亚硝基对细胞的氧化损伤、防止细胞凋亡。The cytotoxic effect of peroxynitrite on cerebellar granule neurons was studied. Exposure of cerebellar granule neurons to 10 μmol/L peroxynitrite triggered apoptosis in vitro, which was confirmed by both morphological(nuclear morphology observed by fluorescence microscopy) and biochemical evidence(DNA fragmentation detected by ELISA). Using ESR spin labeling technique, the alteration of biophysical characteristics of neuronal cell membrane during the apoptotic process was studied. The results indicate that after treatment with peroxynitrite, the fluidity of both the surface layer and the deep layer of the neuronal cell membrane decreased markedly, and the S/W ratio of the membrane protein thiol groups increased significantly. Pre treating cerebellar granule neurons with antioxidant EPC K1, a novel water soluble derivative of vitamin C and vitamin E, alleviated the oxidative injury and prevented cells from apoptosis.

关 键 词：小脑 颗粒神经元 细胞凋亡 过氧化亚硝基 

分 类 号：R977.2[医药卫生—药品] R741.05[医药卫生—药学]