O_2^-·与红细胞膜阴离子转运蛋白相互关系的研究  

Relationship between Superoxide Anion Radical and Anion Transport Protein of Red Blood Cell Membrane

作　　者：符乃阳[1,2] 张尔贤[1,2] 林哲甫 俞丽君[1,2] 

机构地区：[1]汕头大学理学院生物学系 [2]中山大学生命科学学院生物化学系

出　　处：《生物化学与生物物理学报》1999年第3期328-330,共3页

摘　　要：利用４，４′二硫氰２，２′二磺基芪（ＤＩＤＳ）对带３（ｂａｎｄ３）蛋白阴离子转运功能的专一抑制作用，研究了超氧阴离子（Ｏ－２·）进入红细胞对带３蛋白的依赖关系；同时根据红细胞在等渗氯化铵溶液中溶血的ｔ１／２，探讨了细胞外大量产生Ｏ－２·对带３蛋白转运阴离子功能的影响。结果表明：４０μｍｏｌ／ＬＤＩＤＳ完全抑制带３蛋白的阴离子转运功能，但并不影响Ｏ－２·进入细胞，证明Ｏ－２·进入细胞对ｂａｎｄ３蛋白无依赖关系；Ｏ－２·通过氧化修饰红细胞膜蛋白的－ＳＨ，使带３蛋白的阴离子转运能力下降，并可用－ＳＨ还原剂ＤＴＴ与巯基乙醇修复。By using a specific inhibitor of anion transport, DIDS, the dependence on band 3 anion transport protein of superoxide anion radical (O - 2·) flux across red blood cell (RBC) membrane into RBC was studied; and the effect of superoxide anion radical on anion transport in RBC membrane was also discussed based on the hemolytic t 1/2 in an isotonic solution of NH 4Cl. The results showed that 40 μmol/L DIDS could completely inhibit anion transport by band 3 protein in RBC membrane, but had no effect on flux into RBC, proving that the O - 2· transport into RBC across RBC membrane was independent of band 3 protein; the interaction of O - 2· with specific SH group of membrane protein lowered the anion permeability, and it could be reversed by DTT and mercaptoethanol.

关 键 词：超氧阴离子 带3蛋白 红细胞 转运蛋白 

分 类 号：Q592.1[生物学—生物化学]