人类β-arrestin1 cDNA的克隆、表达及功能的研究  

Molecular Cloning of Human β arrestin1 cDNA, Expression and Functional Study

作　　者：郁庆明[1,2] 程智洁[1,2] 周天华 岑博[1,2] 郭盛淇 裴钢 

机构地区：[1]中国科学院上海细胞生物学研究所 [2]卫生部上海生物制品研究所

出　　处：《生物化学与生物物理学报》1999年第3期344-346,共3页

摘　　要：用生物信息法获得了人类βａｒｅｓｔｉｎ１两个亚型（１Ａ和１Ｂ）的全长编码序列的ｃＤＮＡ。βａｒｅｓｔｉｎ１Ａ可以在大肠杆菌中高效表达，部分产物以包涵体形式存在。利用纯化的包涵体在小鼠中制备了抗血清。免疫印迹法分析表明，βａｒｅｓｔｉｎ１Ａ也可以在人胚胎肾２９３细胞中表达．对其功能的研究证明，βａｒｅｓｔｉｎ１Ａ可以强烈削弱共表达的阿片受体对抑制性Ｇ蛋白的激活。Complete coding sequences of β arrestin1 (1A and 1B) were cloned through application of bioinformatics analysis to the dbEST database. β arrestin1A was overexpressed in E.coli with partial expression products as inclusion body. Anti β arrestin1 antibodies were prepared by using purified inclusion body. Results also demonstrate that activation of inhibitory G protein mediated by δ and κ pioid receptors was strongly attenuated by overexpression of β arrestin1A in co transfected 293 cells.

关 键 词：Β-ARRESTIN 表达 抑制性G蛋白 CDNA 克隆 功能 

分 类 号：Q523[生物学—生物化学] Q74