甲状旁腺素、胰岛素样生长因子-Ⅰ对骨特征性标志蛋白的影响  

The Effect of Parathyroid Hormone,Insulin like Growth Factor Ⅰ On Bone Marker Proteins

作　　者：白秀英[1] 俞文华[1] 李平风[1] 周爱儒[1] 顾文霞[1] 杜国光[1] 

机构地区：[1]北京医科大学生物化学与分子生物学系

出　　处：《中国生物化学与分子生物学报》1999年第2期300-303,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金

摘　　要：为了阐明甲状旁腺素（ｐａｒａｔｈｙｒｏｉｄｈｏｒｍｏｎｅ，ＰＴＨ）、胰岛素样生长因子－Ⅰ（ｉｎｓｕｌｉｎ－ｌｉｋｅｇｒｏｗｔｈｆａｃｔｏｒ－Ⅰ，ＩＧＦ－Ⅰ）在促成骨样细胞ＲＯＳ１７／２．８增殖的同时对其分化状况的影响，研究了ＰＴＨ、ＩＧＦ－Ⅰ对骨特征性标志蛋白碱性磷酸酶（ａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ，ＡＬＰ）活性、骨钙素（ｏｓｔｅｏｃａｌ－ｃｉｎ）ｍＲＮＡ表达及骨胶原蛋白（ｃｏｌａｇｅｎ）合成的影响．结果表明，ＰＴＨ、ＩＧＦ－Ⅰ均可抑制ＡＬＰ活性，ＰＴＨ的抑制作用强于ＩＧＦ－Ⅰ，抑制作用具有时间剂量依赖性；ＰＴＨ、ＩＧＦ－Ⅰ均可诱导骨钙素ｍＲＮＡ表达增加；ＰＴＨ能够促进３Ｈ－脯氨酸参入新生小鼠颅骨组织，既ＰＴＨ能够骨胶原蛋白合成增加．这些结果提示，ＰＴＨ、ＩＧＦ－Ⅰ在促成骨样细胞ＲＯＳ１７／２．８增殖的同时也促进其分化，表明在成骨细胞的生发成熟过程中，增殖、分化现象相辅相成，相伴而行．Parathyroid hormone(PTH)plays an important role in regulation of calcium and phosphorus homeostasis.Recently it has been demonstrated that PTH can also enhance hone anabolism.In osteoblast like ROS 17/2 8 cells the proliferative effect of PTH has already been observed.This report describes the differentiative effects of PTH(10 -9 and 10 -10 mol/L)that down regulated alkaline phosphates activity,increased the expression of osteocalcin mRNA,and enhanced the incorporation of 3 H proline into collagen.Similar results were also demonstrated in IGF Ⅰ treated ROS 17/2 8 cells.Alkaline phosphatase,osteocalcin and collagen are three main marker proteins of differentiated osteoblast.It means that PTH and IGF Ⅰ can both regulate proliferation and differentiation at different stages of cell growth in osbteoblast like ROS 17/2 8 cells.

关 键 词：甲状旁腺素 IGF-1 碱性磷酸酶 骨钙素 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] Q573[医药卫生—基础医学]