滞育诱导温度和光照节律对家蚕sod基因和cat基因的表达的影响  

Effect of Diapause-inducing Temperature and Photoperiod on the Expression of gene sod and cat of Silkworm,Bombyx Mori

作　　者：陶卉[1] 李茜[1] 徐丽[1] 季明明[1] 徐世清[1,2] 

机构地区：[1]苏州大学医学部应用生物学系,江苏苏州215123 [2]苏州大学现代丝绸国家工程实验室

出　　处：《江苏蚕业》2010年第3期6-12,共7页Jiangsu Sericul Ture

基　　金：国家自然科学基金项目(No.30771632);国家科技支撑计划重点项目(No.2007BAD72B01);苏州大学重大应用研究培育项目(No.Q3034850)

摘　　要：家蚕(Bombyxmori)是卵滞育性鳞翅目模式昆虫,其滞育性受亲代卵孵化期(催青期)温度、光节律等环境因子诱导。为了研究家蚕滞育与温度和光照节律的关系,本文调查了温度、光照节律对家蚕sod基因和cat基因表达的影响。催青期中除了孵化期,25℃持续光照下(25LL)卵的sodmRNA均保持在较高水平,另外,sod基因在20℃光照黑暗(20LD)12h交替环境下的表达量要高于15℃持续黑暗(15DD)条件,且孵化后期这种现象更加明显。cat基因在25LL、20LD和15DD环境之间的表达量无差异,但是孵化后期(积温2160-3600℃.h),cat基因表达水平显著上调,这与在此阶段的蚕卵胚胎对滞育诱导环境温度和光节律比较敏感的结论相一致。此外,本文还调查了子代卵中sod基因和cat基因的表达情况。sod基因在人工活化卵(DTE)中的表达水平显著高于非浸酸的滞育性卵(DDE);卵龄24-192h,25LL子代卵逐渐进入滞育,sod基因表达量下调。低温黑暗条件会上调家蚕子代卵sod基因的表达水平。在初期的盐酸活化卵(DTE)中,cat表达量低于滞育卵(DDE),而后逐渐上升,直至接近滞育卵表达量,这表明盐酸可能会抑制cat基因表达。在恒温条件下,黑暗条件会促进子代卵上调cat表达。在温度光照协同作用下,20LD子代卵中cat表达量低于25LL和15DD。Bombyx mori is the model insect of lepioloptera with diapausing during its egg stage. Diapause inducements are due to temperature and photoperiod in the incubation process of parental eggs. to investigate the relationship of diapause inducements and temper- ature and photoperiod, we design this experiment to search the affect of temperature and photoperiod on the gene sod and cat. When eggs were incubated in 25 LL, their sod mRNA would keep at a high level, but the transcription level was rapidly down-regulated at EAT1 800~C. h and 3 600℃. h. The expression of sod gene in 20 LD was significantly higher than in 15 DD, this phenomenon was more obvious when the embryonic nervous had fully grown in the later incubation stage. The expression of cat gene had no obvious differences when eggs were incubated in 25 LL, 20 LD or 15 DD but it was notable that the mRNA level of cat all increased in the late incubation stage, which was in accordance with the fact that the eggs were more sensitive to diapause-inducing incubation temperature and photoperiod of this time. ＇After artificial hatching treatment with hydrochloric acid at 24h after oviposition, the sod mRNA in dia- pause-tetrainated eggs （DTE） was much higher than diapause-destined eggs （DDE）. From 24 to 192hours after oviposition, eggs at progeny went into the diapause stage, the transcription level of sod subsequently decreased. After we examined the common effect of il- lumination and temperature on the sod gene of progeny eggs, it was observed that the sod mRNA increased under constant darkness at 15~C. The cat mRNA level of DTE was lower than DDE at first after oviposition, but then the mRNA increased, which indicated that the expression of cat might be inhibited by hydrochloric acid at the early activation. Then we investigated the common effect of illumination and temperature on the cat gene of progeny eggs and found that the expression level of cat in 20 LD was lower than in 25 LL and 15 DD.

关 键 词：家蚕 滞育 温度 光照节律 基因表达 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]