低剂量敌百虫对拟环纹豹蛛体内酯酶同工酶的影响  

Effect of Low Dose of Trichlorfon Pesticide on Esterase Isozymes in the Pardosa pseudoannulata

作　　者：廖艳阳[1] 张祖娇[2] 王智[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南科技学院,湖南永州425902

出　　处：《湖南农业科学》2010年第9期82-84,共3页Hunan Agricultural Sciences

基　　金：湖南省科技厅项目资助(2009RS3009)

摘　　要：运用聚丙烯酰胺凝胶垂直板型电泳技术,对有敌百虫农药作用下不同发育时期拟环纹豹蛛体内酯酶同工酶进行了比较研究,结果发现不同发育时期酯酶同工酶有着较大的差异,酶带数呈现出少-多-少的趋势,6龄期达到峰值。且农药胁迫下拟环纹豹蛛的酯酶同工酶的酶带增多,酶浓度增大。A comparative study was conducted to the esterase isozymes in Pardosa pseudoannulata at different growth periods under application of trichlorfon pesticide by using the vertical slab polyacrylamide gel electrophoresis technology.The results showed that there were significant differences between esterase isozymes in different growth periods;the number of isozyme band showed a trend of less-more-less,and it reached peak at 6th instar;the concentration of isozymes increased with the increasing number of isozyme band in Pardosa pseudoannulata under the stress of trichlorfon pesticide.

关 键 词：敌百虫农药 拟环纹豹蛛 酯酶同工酶 

分 类 号：S646.19[农业科学—蔬菜学]