CO_2/空气比值对牟氏角毛藻生长和总脂含量的影响  

Effects of Different CO_2/Air Ratios on the Growth and Total Lipid Content of Chaetoceros muelleri

作　　者：王蒙[1,2] 李纯厚[1] 戴明[1] 韦芳三[1,2] 周岩岩[1,2] 杨慧英[1,2] 胡维安[1,2] 杨进[1,2] 

机构地区：[1]中国水产科学研究院南海水产研究所农业部海水养殖生态与质量控制重点开放实验室,广东广州510300 [2]上海海洋大学,上海201306

出　　处：《湖南农业科学》2010年第9期134-138,共5页Hunan Agricultural Sciences

基　　金：广东省海洋渔业科技推广专项(A200899I04)

摘　　要：以牟氏角毛藻作为试验材料,通过向培养液中通入5种不同比值的CO2与空气的混合气体培养牟氏角毛藻,研究了CO2/空气比值对海洋产能微藻的生长以及总脂含量的影响。结果显示:CO2/空气的比值对牟氏角毛藻的相对生长率、总脂含量、总脂日增量均有极显著影响;牟氏角毛藻在CO2/空气=1/15时相对生长率最高,为1.76 d-1;总脂含量百分比最高,为31.26%;总脂日增量也最高,为样品藻初始干重的0.75倍。牟氏角毛藻的总不饱和脂肪酸的含量随CO2/空气比值的减小而增加,CO2/空气=1/25时最高,达70.27%;CO2/空气=1/5时最低,为65.28%。研究表明,CO2/空气=1/15的混合气体最适合牟氏角毛藻的生长以及粗脂肪的积累;CO2/空气=1/25最适合牟氏角毛藻不饱和脂肪酸的积累。The effects of the different CO2 /air ratios on the growth and total lipid content of Chaetoceros muelleri were studied by adding gas mixture with five different CO2 /air ratios to the culture medium of Chaetoceros muelleri.The results showed that the CO2 /air ratio has extremely effect on relative growth rate,total lipid content and daily increment of total lipid of Chaetoceros muelleri;when CO2 /air ratio was 1/15,the relative growth rate of Chaetoceros muelleri was the highest with K=1.76 d-1,the percentage of total lipid content of it was also the highest with P=31.26%,and the daily increment of total lipid still was the highest with Q=0.75;the content of the total unsaturated fatty acid of Chaetoceros muelleri increased with decreasing CO2 /air ratio,when CO2 /air ratio was 1/25,it reached the highest with 70.27%,when CO2 /air ratio was 1/5,it was the lowest with 65.28%.This study showed that the CO2 /air ratio of 1/15 is the optimum ratio for the growth and the crude-fat accumulation of Chaetoceros muelleri,and the CO2/air ratio of 1/25 is the optimum ratio for the accumulation of unsaturated fatty acids of Chaetoceros muelleri.

关 键 词：牟氏角毛藻 CO2/空气比值 总脂含量 相对生长率 总脂日增量 

分 类 号：S968.4[农业科学—水产养殖]