HPLC法测定十滴水中五种活性成分的含量  

Determination of five effective constituents in Shidishui by HPLC

作　　者：陈广通[1] 李玉琴[1] 黄金华[1] 汪冬庚[1] 王新杨[1] 

机构地区：[1]南通大学医学院,江苏南通226001

出　　处：《中国医药导报》2010年第29期40-42,共3页China Medical Herald

摘　　要：目的:建立HPLC法同时测定十滴水中的芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法。方法:色谱柱为ShimadzuVP-ODS柱(4.6mm×250mm,5μm),流动相为乙腈-0.1%磷酸梯度洗脱,流速为1.0ml/min,检测波长为254nm,柱温30℃。结果:5种成分分别在0.042～0.840μg(r=0.9996)、0.168～3.352μg(r=0.9998)、0.084～1.680μg(r=0.9997)、0.093～1.852μg(r=0.9999)和0.174～3.490μg(r=0.9994)内呈良好线性关系,平均回收率分别为99.60%(RSD=0.20%)、99.56%(RSD=0.36%)、98.95%(RSD=0.55%)、98.76%(RSD=0.97%)和98.79%(RSD=1.08%)。结论:本法简便、快速、准确,可用于十滴水的质量控制。Objective： To establish a method for the simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Shidishui by HPLC. Methods： The sample was separated on a Shimadzu VP-ODS （4.6 mm×250 mm, 5.0 μm） with acetonitrile-0.1% phosphoric acid in gradient elution. The flow rate was 1.0 ml/min, the detection wavelength was 254 nm and the column temperature was 30℃. Results： Aloe-emodin, rhein, emodin, chrysophanol and physcion had good linear relationship in the range of 0.042-0.840 μg （r=0.999 6）, 0.168-3.352 μg （r=0.999 8）, 0.0841.680 μg （r=0.999 7）, 0.093-1.852 μg （r=0.999 9） and 0.174-3.490 μg （r=0.999 4）. Their average recoveries were 99.60% （RSD=0.20%）, 99.56% （RSD=0.36%）, 98.95% （RSD=0.55%）, 98.76% （RSD=0.95%）, and 98.79% （RSD=1.08%）, respectively. Conclusion： The method is rapid, simple, accurate, and applicable for the quality control of Shidishui.

关 键 词：十滴水 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 高效液相色谱 

分 类 号：R642.5[医药卫生—外科学]