RP-HPLC测定宫瘤消片中芍药苷的含量  

Content determination of Peoniflorin in Gongliuxiao Tablets by RP-HPLC

作　　者：张迪 马晓璐 张国刚[2] 

机构地区：[1]辽宁省辽阳市药品检验所,辽宁辽阳111000 [2]沈阳药科大学,辽宁沈阳110016

出　　处：《中国医药导报》2010年第29期44-46,共3页China Medical Herald

摘　　要：目的:建立高效液相法测定宫瘤消片中芍药苷含量的方法。方法:采用Agilent-C18(4.6mm×150mm,5μm)色谱柱;流动相:乙睛-0.1%磷酸溶液(15∶85);检测波长:230nm;流速:1.0ml/min。结果:替加氟在0.2～1.0μg范围内与峰面积呈良好的线性关系(r=0.9992),平均回收率为99.26%,RSD=1.65%(n=5)。结论:本方法简便、准确、重现性好,可用于宫瘤消片中芍药苷的质量控制。Objective： To establish a method for the content determination of Peoniflorin in Gongliuxiao Tablets by HPLC. Methods： The Agilent-C18 （4.6 mm×150 mm, 5 μm） column was used. The mobile phases consisted of acetonitrile and 0.1%-phosphoric acid （15∶85）, the flow rate was 1.0 ml/min and the detection wavelength was at 230 nm. Results： The linear ranges of Peoniflorin was at 0.2-1.0 μg （r=0.999 7）, the average recovery was 99.70% with a RSD of 1.65% （n=5）. Conclusion： The method is fast, reliable, accurate and can be used in the quality control of Peoniflorin in Gongliuxiao Tablets.

关 键 词：宫瘤消片 芍药苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]