柚皮中柚皮甙的提取纯化及结构鉴定  

Extraction,purification of naringin from pomelo peel and structure identification

作　　者：叶翠层[1] 周尽花[1] 吴宇雄[1] 孙汉洲[1] 

机构地区：[1]中南林业科技大学应用化学研究所,湖南长沙410004

出　　处：《中南林业科技大学学报》2010年第10期121-124,共4页Journal of Central South University of Forestry & Technology

基　　金：中南林业科技大学引进人才项目(104-0071)

摘　　要：从柚皮中提取分离纯化活性物质柚皮甙可以促进柚果资源的高效利用。采用乙醇-水体系从柚皮中提取了柚黄酮类化合物并采用大孔吸附树脂法对其进行纯化。采用熔点、紫外、红外、核磁共振氢谱和质谱对精制的柚黄酮单体进行检测分析。实验结果表明:乙醇-水体系和大孔吸附树脂法可以有效地实现柚黄酮化合物的提取和纯化;熔点及四大谱图确证了分离纯化精制得到的柚黄酮单体为柚皮甙。Separation and purification of bioactive component naringin from pomelo（Citrus grandis Osbeck） peel can improve the efficient use of pomelo resource.Flavonoids were extracted by ethanol-water solution from pomelo peel and purified by porous resin adsorption method.Ultraviolet（UV）,infrared（IR）,1H nuclear magnetic resonance（1H NMR）,mass spectrometry（MS） and melting point（m.p.） method were employed to characterize the obtained flavonoid monomer.Experimental results show that ethanol-water extraction system and porous resin adsorption are effective for the separation and purification of flavonoid.UV,IR,1H NMR,MS and m.p.has confirmed that the flavonoid monomer is naringin.

关 键 词：柚皮甙 提纯 鉴定 黄酮 柚 

分 类 号：O629.9[理学—有机化学]