硫酸钙/纳米羟基磷灰石构建人工骨材料的实验研究  

Study of experiment about Calcium sulfate/nano hydroxyapatite constructing artificial bone material

作　　者：席焱海[1] 叶晓健[1] 葛亮[1] 杨四川[2] 欧阳跃平[1] 刘岩[1] 苟三怀[1] 

机构地区：[1]上海长征医院,上海200003 [2]上海同济大学,上海200092

出　　处：《生物骨科材料与临床研究》2010年第5期1-5,共5页Orthopaedic Biomechanics Materials and Clinical Study

摘　　要：目的探索将纳米羟基磷灰石(n-HA)/硫酸钙与半水硫酸钙(2CaSO4·H2O,CSH)组合构建一种新型的人工骨材料,研究其降解速度、理化特性及物质的变化过程,并对该变化与构建人工骨材料的成骨活性的相互作用及关系进行分析。方法模拟人体体液SBF9#浸泡液,将5组样品进行浸泡测试;5组分别是:n-HA、CSH、CSH/10wt%HA、CSH/20wt%HA和CSH/40wt%HA,测定钙磷元素释放速率、失重率及CSH/HA变化并对复合人工骨材料表面形态作扫描电镜观察和XRD分析。结果体外浸泡后,n-HA在24h内质量失重50%,24h后基本稳定;CSH外形及失重变化稳定,但2周后仅剩余少量,到17天时完全降解;3组复合材料质量失重及外形变化不大。5组在浸泡后24h内均有Ca释放,但n-HA组的Ca释放低于其他4组,各组均有P元素释出,但随时间逐渐减少。XRD结果分析:复合人工骨材料浸泡中的峰值下降,HA峰值强度增高。电镜观察到浸泡后复合材料形成新的晶体形态。结论本研究将硫酸钙、纳米羟基磷灰石2种材料复合,其主要成分存在相互作用,在模拟人体体液浸泡的环境下出现表面Ca、P元素的释放-吸附动态过程并新生磷灰石层,材料的降解速度可通过调节成分配比而加以控制。Objective To explore the Nano-hydroxyapatite（n-HA）/calcium sulfate and（2CaSO4·H2O,CSH） to construct a new combination of artificial bone material,and study the degradation rate,physical and chemical properties and physical changes,and analyze the interaction and the relationship between this change and bone-inducing activity of process of constructing artificial bone.Methods Samples of n-HA、CSH、CSH/10wt%HA、CSH/20wt%HA and CSH/40wt %HA materials incorporating different amount of HA were soaked into SBF9# solutions.Calcium and Phosphorus element concentration of solutions were measured.Daily losing mass of all samples.Scaning electron microscope observation and XRD analysis were also performed.Results After soaked into SBF9# solutions out of the body,sample of n-HA underwent 50% weight losing,and kept basically stable after first 24 hours.CSH was stable in appearance and weightlessness losing,but a few left after two weeks,and completely degradation in the 17s day.The changes of three groups of composite material＇s weightlessness and appearance were small.Five groups after soaking in released Ca in first 24hours,but lower Ca concentration was measured in solutions for n-HA group.Phosphorus content was released from all groups and decreased when soaking time lengthened.XRD indicated that the diffractive waves of HA enhanced in composite material samples,while SEM showed new crystal formation on their surfaces.Conclusion We study the interaction between n-HA and CS composite material,and find they may took place a dynamic equilibrium of Ca、P releasing-adsorpsion thus occurred on material surfaces and generated a new apatite layer formed.The degradation rate of composite could be controlled by regulating it＇s component proportions.

关 键 词：硫酸钙 纳米羟基磷灰石 人工骨材料 

分 类 号：R318.08[医药卫生—生物医学工程]