TSBAb-ELISA法的建立与临床初步应用  

The Establishment of TSBAb-ELISA and Its Preliminary Utilization for Clinic

作　　者：陈宁[1,2] 汤特[1,2] 张天庚[1,2] 叶静 安桂珍[1,2] 张宝泉[1,2] 

机构地区：[1]天津医科大学病理生理学教研室 [2]天津医科大学总医院核医学科

出　　处：《标记免疫分析与临床》1999年第2期101-104,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：利用杂交瘤技术制备了抗人甲状腺刺激阻断抗体（ｔｈｙｒｏｉｄ－ｓｔｉｍｕｌａｔｉｏｎｂｌｏｃｋｉｎｇａｎｔｉｂｏｄｉｅｓ，ＴＳＢＡｂ）单克隆抗体。两株单抗均为ＩｇＧ１，识别同一抗原表位。与甲状腺球蛋白（ｔｈｙｒｏｇｌｏｂｕｌｉｎ，ＴＧ）、甲状腺过氧化酶（ｔｈｙｒｏｉｄｐｅｒｏｘｉｄａｓｅ，ＴＰＯ）无交叉反应，与促甲状腺激素受体抗体（ｔｈｙｒｏｔｒｏｐｉｎｒｅｃｅｐｔｏｒａｎｔｉｂｏｄｉｅｓ，ＴＲＡｂ）、甲状腺刺激抗体（ｔｈｙｒｏｉｄｓｔｉｍｕｌａｔｉｎｇａｎｔｉｂｏｄｉｅｓ，ＴＳＡｂ）有部分交叉反应。用间接ＥＬＩＳＡ法检测甲减患者血清ＴＳＢＡｂ水平，表明该法有一定特异性、灵敏、稳定、简便。利用抗人ＴＳＢＡｂ单抗和ＥＬＩＳＡ技术，可为临床检测ＴＳＢＡｂ和探讨自身免疫性甲状腺机能低下的发病机理提供一种新的手段。The monoclonal antibodies against human thyroidstimulation blocking antibodies(anti-TSBAb McAb) were prepared by hybridoma technique.Two McAb are both IgG1 and recognized a same epitope.The McAb hadnt cross-reactivity to TG and TPO,but had partial cross-reactivity to TRAb and TSAb.The level of TSBAb in hypothyroidism patients aera was detected by indirect ELISA.Results showed that this method has higher sensitivity and stability,and was simple.Utilization of anti-TSBAb McAb and ELISA technique may provide a method for detecting TSBAb and reseaching pathogenesis of autoimmune hypothyroidism.

关 键 词：甲状腺刺激 阻断抗体 ELISA 甲状腺功能减退 

分 类 号：R446.61[医药卫生—诊断学] R581.202[医药卫生—临床医学]