内源性高甘油三酯血症患者载脂蛋白E多态性及血脂、载脂蛋白的含量  

Apolipoprotein E Polymorphism, Plasma Lipids and Apolipoproteins in Chinese Endogenous Hypertriglyceridemia

作　　者：范平[1,2] 刘宇[1,2] 张祖辉[1,2] 刘秉文 顾华强[1,2] 

机构地区：[1]华西医科大学基础医学院载脂蛋白研究室 [2]华西医科大学附属第四医院检验科

出　　处：《中国动脉硬化杂志》1999年第2期117-119,共3页Chinese Journal of Arteriosclerosis

基　　金：卫生部科研基金

摘　　要：为探讨内源性高甘油三酯血症患者载脂蛋白Ｅ多态性及其与血脂和载脂蛋白水平的关系，采用等电聚焦免疫印迹分析法、酶法及单向免疫扩散法，分别对内源性高甘油三酯血症患者及健康受试者的载脂蛋白Ｅ基因型、表型、空腹血脂及载脂蛋白ＡＩ、Ｂ１００、ＣⅡ、ＣⅢ进行了全面分析。结果发现，与对照组比较，内源性高甘油三酯血症患者空腹血清甘油三酯、总胆固醇、载脂蛋白Ｂ１００、ＣⅡ、ＣⅢ、Ｅ水平明显升高（Ｐ＜０．００１），载脂蛋白ＡＩ明显降低（Ｐ＜０．００１）；对照组与内源性高甘油三酯血症组载脂蛋白Ｅ表型及等位基因频率分布以Ｅ３／３和ε３最高，对照组载脂蛋白Ｅ２亚组血清甘油三酯及载脂蛋白Ｂ１００水平较载脂蛋白Ｅ３或Ｅ４亚组降低（Ｐ＜０．０５），载脂蛋白Ｅ水平升高（Ｐ＜０．０５）；而在内源性高甘油三酯血症组并未发现类似改变（Ｐ＞０．０５）。以上提示，在正常对照组中，ε２可引起低总胆固醇及高载脂蛋白Ｅ水平，从而发挥抗动脉粥样硬化的作用，而内源性高甘油三酯血症患者该保护作用明显下降或消失。Aim To study apolipoprotein (apo) E polymorphism and its relationship with plasma lipids and apolipoproteins in Chinese patients with endogenous hypertriglyceridemia(HTG). Methods ApoE phenotype was assayed by isoelectric focusing (IEF) and immunoblotting, serum lipids were determined by enzyme method and apolipoproteins were measured by radial immunodiffusion assay in 93 endogenous HTG patients whose fasting serum TG levels were ≥2.26 mmol/L, TC<6.21 mmol/L and 288 healthy subjects whose fasting serum TG levels were<1.82mmol/L, TC<6.21 mmol/L. Results The fasting serum TG, TC, apoB100, CⅡ, CⅢ and E levels were significantly higher and apoAI level was lower in the endogenous HTG group than those in the control group (P<0.001). ApoE phenotype and allele frequency distribution of the endogenous HTG group (E3/3 68.82%, E2/3 17.20%, E3/4 9.68%, E2/4 4.30%, E4/4 0.00%, E2/2 0.00%, ε3 0.8226, ε2 0.1075, ε4 0.0699)was similar to that of the control group( E3/3 72.92%, E2/3 13.54%, E3/4 10.76%, E2/4 1.04%, E4/4 1.74%, E2/2 0.00%, ε3 0.8507, ε2 0.0729, ε4 0.0764). Compared with apoE3(E3/3) or apoE4(E3/4+E4/4) subgroup, apoE2(E2/3+E2/2) was associated with lower serum TC and apoB100 levels (P<0.05) and higher apoE level (P<0.05) in the control group, but no significant difference was observed in endogenous HTG group. Conclusion The apoE phenotype and allele frequency distribution of the endogenous HTG group was similar to that of the control group. ApoE2 was associated with lower serum TC and apoB100 levels and higher apoE level in the control group.

关 键 词：高甘油三酯血症 载脂蛋白E 血脂 载脂蛋白 

分 类 号：R589.202[医药卫生—内分泌]