百草枯中毒致大鼠肺纤维化的实验研究  

Study on pulmonary fibrosis induced by acute paraquat poisoning in rats

作　　者：王伟[1] 宋祖军[1] 段小红[2] 孟焕成[1] 马俊清[1] 王玉挺[1] 种剑[2] 

机构地区：[1]西安第四军医大学西京医院急诊科,710032 [2]西安第四军医大学口腔医院中心仪器室

出　　处：《中国急救医学》2010年第9期818-821,867,共5页Chinese Journal of Critical Care Medicine

摘　　要：目的 探讨急性百草枯（PQ）中毒对大鼠肺纤维化的作用机制,为防治肺纤维化提供依据.方法 56只SD大鼠随机分为对照组和染毒组,染毒组32只,对照组24只,染毒组以4 mg/mL浓度的百草枯稀释溶液按14 mg/kg剂量一次性腹腔注射,对照组代以等剂量生理盐水注射.选取染毒组12只、对照组4只分别于第3、7、14、28天行活体肺组织Micro CT扫描,剩余大鼠分别于上述4 d每组各处死5只.处死剖胸后完整取出肺组织并观察整体肺的变化情况;称质量肺组织计算肺系数;通过HE染色观察肺组织的病理改变;测定肺组织匀浆中羟脯氨酸（HYP）的含量变化.结果 大鼠染毒后均出现行为异常;光镜及Micro CT观察肺组织可见随染毒时间的延长肺泡炎及肺纤维化征象相继发生;染毒组大鼠肺系数在各时间段皆高于相应对照组,差异有统计学意义（P〈0.05或P〈0.01）,且染毒组内随染毒时间的延长肺系数亦显著升高（P〈0.01）;染毒组大鼠肺组织HYP含量在第7、14、28天时皆高于相应对照组,差异有统计学意义（P〈0.01）,且染毒组内随染毒时间的延长HYP含量亦显著增长（P〈0.01）.结论 百草枯中毒可导致大鼠肺组织发生纤维化,羟脯氨酸含量变化可判断纤维化程度,Micro CT可用于肺纤维化的检测.Objective To explore the pathogenesis of pulmonary fibrosis in rats with acute paraquat （PQ） poisoning,in order to provide the basis for controlling pulmonary fibrosis. Methods 56 male Sprague Dawley rats were randomly divided into control group （24） and paraquat poisoned group （32）. The rats in exposure group were treated with dilute solution of paraquat （4 mg/mL） at 14 mg/kg through intraperitoneal injection and the rats in control group were treated with the same volume of saline. 12 rats in poisoned group and 4 rats in control group were taken out to scan lung tissues in vivo by Micro CT on day 3, 7, 14, 28 respectively after administration and others were sacrificed respectively on the four days mentioned above. General situation of the lung, pulmonary index, histological examination and hydroxyproline content were used to determine the severity of pulmonary fibrosis. Results Compared with the control group, the rats with acute paraquat poisoning appeared abnormal symptoms and behaviors. With the extention of exposure time, we found the signs of pulmonary alveolitis and pulmonary fibrosis occurred in succession by optical microscope and Micro CT. Pulmonary indexes of rats in exposure group are higher than corresponding control group （ P 〈 0. 05 or P 〈 0.01 ）, and in poisoned group the pulmonary indexes are significantly upregulated with the extention of exposure time （ P 〈 0. 01 ）. The contents of hydroxyproline of rat＇s lung tissues in exposure group are remarkably higher than corresponding control group on day 7, 14, 28 （ P 〈 0. 01 ）, and in exposure group the pulmonary indexes are also significantly upregulated with the extention of exposure time （ P 〈 0. 01 ）. Conclusion Paraquat poisoning can lead to pulmonary fibrosis in rats, and hydroxyproline content can estimate the severity of pulmonary fibrosis. Micro CT can be used for detection of pulmonary fibrosis.

关 键 词：百草枯 肺纤维化 肺系数 羟脯氨酸 MICRO CT 

分 类 号：R595.4[医药卫生—内科学]