负载胰岛素壳聚糖接枝醋酸乙烯酯纳米粒的制备和性能  

Preparation and properties of chitosan-graft-vinyl acetate copolymer nanoparticles loaded with insulin

作　　者：刘占军[1,2] 郭启仓[3] 于九皋[2] 韩刚[1] 

机构地区：[1]华北煤炭医学院药学系,河北唐山063000 [2]天津大学理学院,天津300072 [3]华北煤炭医学院附属医院,河北唐山063000

出　　处：《中国医院药学杂志》2010年第18期1517-1521,共5页Chinese Journal of Hospital Pharmacy

摘　　要：目的:制备负载胰岛素壳聚糖-醋酸乙烯酯共聚物纳米粒并研究其性能。方法:用自由基聚合法合成壳聚糖-醋酸乙烯酯共聚物,该聚合物在水中形成具有疏水核心、亲水表面的纳米粒。采用正交设计试验研究了投料比、引发剂浓度和反应时间对纳米粒粒径的影响。结果:对纳米粒进行了热重分析和红外表征。测定纳米粒的形态、粒径和表面电位(Zeta电位),以胰岛素为模型药物,研究纳米粒的包封和释药性能。结论:纳米粒呈球形,粒径均匀,表面荷正电。胰岛素的包封率可达90%以上。pH6.8的磷酸盐缓冲液中胰岛素释放较慢。结论:该纳米制剂具有较好的物理性能和体外缓释特性。OBJECTIVE To prepare and study the properticles of the chitosan-graft-poly（vinyl acetate） copolymernanoparticles loaded with insulin. METHODS The chitosan-graft-poly（vinyl acetate） copolymers were synthesized by free radical polymerization, and the copolymer formed the nanoparticles, consisted of hydrophobic core and hydrophilic surface. The effect of feed ratio of vinyl acetate to chitosan the initiator concentration and reaction time on the particle size of nanoparticales was investigated by the orthogonal test method. The grafted products were characterized by thermogravimetric analysis （TGA） and FTIR. The morphology, particle size and zeta potential of nanoparticles were evaluated. Using insulin as the model drug, the encapsulation capability of the nanoparticles and insulin release were studied. RESULTS The nanoparticles were discrete and uniform spheres, covered with positive charges. The encapsulation efficiency of nanoparticles was up to 90 %. The in vitro release profile showed the slower release rate of insulin in phosphate buffer at pH 6. 8. CONCLUSION The nanoparticles possessed good physical performance and sustained release character in vitro.

关 键 词：壳聚糖 醋酸乙烯酯 纳米粒 包封率 体外释放 

分 类 号：R943[医药卫生—药剂学]