合欢叶提取物对油菜菌核病菌的抑制作用及其苗期防治效果  

Inhibition of extracts from the leaves of Albizzia julibrissin to Sclerotinia sclerotiorum and control effect at the seedling stage

作　　者：刘峰[1] 代光辉[1] 周熙荣[2] 陈佳[1] 李会[1] 

机构地区：[1]上海交通大学农业与生物学院农业部都市农业(南方)开放重点实验室,上海200240 [2]上海市农业科学院作物育种栽培研究所,上海201106

出　　处：《植物病理学报》2010年第5期511-516,共6页Acta Phytopathologica Sinica

基　　金：国家科技部农业科技成果转化基金(083919n8100);上海市蔬菜学重点学科基金(B209)

摘　　要：不同浓度的合欢叶提取物对油菜菌核病菌(Sclerotinia sclerotiorum)菌丝生长及菌核形成的抑制试验表明,该提取物具有抑制菌丝生长及菌核形成的作用,且随着浓度的提高活性增强,当浓度为20 mg/mL时,对菌丝生长的抑制率为73.1%,对菌核形成的抑制率为100%。通过对提取物处理后菌丝形态显微观察,发现病菌菌丝出现形态异常甚至断裂现象,且培养液电导率增加,推测为提取物处理后菌丝细胞壁破裂、细胞质外渗造成。苗期盆栽试验发现,该提取物对油菜菌核病菌有明显的防治效果,当浓度为20 mg/mL时,防治效果达72.1%。The study showed that the crude extracts from leaves of Albizzia julibrissin Durazz had inhibition effect against the mycelium growth and sclerotial formation of Sclerotinia sclerotiorum in a dose-dependent manner,with the inhibition rate 73.1% for mycelia growth and 100% for sclerotial formation at concentration of 20 mg/mL.The microscope observation showed that the hyphae of S.sclerotiorum were deformed after being treated with the plant extracts,leading to a cytoplasm exudation and the increase of electrical conductivity of the mycelium suspension of the pathogen.The control effect（72.1%） of the extracts（20 mg/mL） against the disease was demonstrated at the seedling stage through pot culture experiment.

关 键 词：合欢 植物提取物 油菜菌核病菌 抑制作用 防治效果 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]