云南甘蔗宿根矮化病调查及种茎温水处理脱菌效果检测  

Investigation of ratoon stunting disease(RSD) on sugarcane in Yunnan and evaluation of the pathogenic bacterium elimination in seed cane by hot-water treatment

作　　者：李文凤[1] 王晓燕[1] 黄应昆 卢文洁[1] 罗志明[1] 

机构地区：[1]云南省农业科学院甘蔗研究所,云南省甘蔗遗传改良重点实验室,开远661600

出　　处：《植物病理学报》2010年第5期556-560,共5页Acta Phytopathologica Sinica

基　　金：现代农业产业技术体系建设专项资金(nycytx-024-01-09);公益性行业(农业)科研专项资助(nyhyzx07-019);云南省“人才培养”项目(2008PY087)

摘　　要：Based on the specific primer from the nucleotide sequences between 16S-23S rDNA,Polymerase chain reaction(PCR) approach for detecting Leifsonia xyli subsp.xyli(Lxx) was established.The approach was applied to detect Lxx from 30 cultivars collected from two main sugar cane producing areas in Yunnan and 10 hot-water treatment samples.The results showed that 80% of the cultivars were infected by Lxx and hot-water treatment was proved to be an efficient measure to control but not completely eliminate Lxx in sugar cane tissues.The PCR products amplified from six infected cultivars were cloned and sequenced,six sequences were acquired and analyzed.It indicated that the six sequences were identical and showed 100% similarity with the isolates from Brazil,Australia and Fujian,and 99.54% with the isolate from Louisiana.Based on the specific primer from the nucleotide sequences between 16S-23S rDNA,Polymerase chain reaction（PCR） approach for detecting Leifsonia xyli subsp.xyli（Lxx） was established.The approach was applied to detect Lxx from 30 cultivars collected from two main sugar cane producing areas in Yunnan and 10 hot-water treatment samples.The results showed that 80% of the cultivars were infected by Lxx and hot-water treatment was proved to be an efficient measure to control but not completely eliminate Lxx in sugar cane tissues.The PCR products amplified from six infected cultivars were cloned and sequenced,six sequences were acquired and analyzed.It indicated that the six sequences were identical and showed 100% similarity with the isolates from Brazil,Australia and Fujian,and 99.54% with the isolate from Louisiana.

关 键 词：甘蔗宿根矮化病 效果检测 温水处理 脱菌 种茎 云南 木质部 澳大利亚 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]