6周无负重游泳运动降低SHR大鼠血压的血浆蛋白质组学研究  

Plasma Proteomics of Six-Week Load-Free Swimming Training Decreasing Blood Pressure in SHR

作　　者：冯红[1] 武晶琼[2] 周春明[2] 何庆华[2] 温利[3] 

机构地区：[1]天津体育学院健康与运动科学系,天津300381 [2]天津体育学院研究生部,天津300381 [3]山西省吕梁市人民医院体检中心,山西吕梁3000031

出　　处：《天津体育学院学报》2010年第5期400-403,共4页Journal of Tianjin University of Sport

基　　金：天津市高等学校科技发展基金项目(项目编号:20060201)

摘　　要：目的:运用比较蛋白质组学技术,探讨6周无负重游泳运动降低SHR大鼠血压血浆蛋白质表达的变化,以期进一步阐述运动治疗高血压病的机制。方法:以雄性4周龄SHR大鼠16只及WKY大鼠16只为研究对象,将其随机分为SHR运动组、SHR安静组、WKY安静组、WKY运动组。SHR运动组及WKY运动组进行1 h/天,5天/周,共6周的无负重游泳运动。使用CODATM2单通道无创血压测量仪检测老鼠血压,对处理好的血浆样品进行二维凝胶电泳和质谱分析。结果:(1)6周无负重游泳运动可以有效降低SHR大鼠的血压。(2)通过质谱分析和数据库检索,成功鉴定了6个差异蛋白:rCG45257、锚定蛋白重复域13家族成员D、甲状腺素转运蛋白A链、Ras相关域1家族同型A、胎球蛋白B前体和γ-肌动蛋白。Objective：Using the comparative proteomics method,the plasma protein expression was examined under the situation of blood pressure decreased by six-week load-free swimming training in spontaneously hypertensive rats（SHR）to further demonstrate the mechanism of hypertension improved by exercise.Methods：Sixteen male four-week old SHR and sixteen Wistar-Kyoto（WKY）rats were randomly divided into SHR exercise group,SHR rest group,WKY exercise group and WKY rest group.Rats in SHR exercise group and WKY exercise group exercised with a six-week load-free swimming protocol（1 hour per day,5 days per week）.The blood pressure of rat was tested by CODATM2 single non-invasive blood pressure measurement appliance.The samples were used to do the two-dimensional gel electrophoresis and mass spectrometry.Results：The blood pressure was decreased significantly after six-week load-free swimming.The six proteins were identified by mass spectrometry and data base retrieval.They are rCG45257,similar to ankyrin repeat domain 13 family,member D,chain A,rat Transthyretin,Ras association domain family 1 isoform A,fetuin B precursor and γ-actin.

关 键 词：6周无负重游泳运动 高血压 血浆蛋白质组学 SHR 

分 类 号：G804.7[文化科学—运动人体科学]