联合应用腹水DNA含量测定鉴别腹水性质的诊断价值  

The Differential Diagnostic Significance of Combind DNA ploidy Analysis in Malignant Ascites

作　　者：武金宝[1] 慧妍[1] 孟宪梅[1] 党彤[1] 

机构地区：[1]包头医学院第二附属医院内蒙古消化病研究所,014030

出　　处：《中国医药指南》2010年第29期181-182,共2页Guide of China Medicine

基　　金：内蒙古自治区高等学校科学研究项目(NJ10183)

摘　　要：目的对腹水DNA含量测定鉴别良、恶性腹水的方法给肿瘤的诊断进行研究。方法随机选取包头医学院第二附属医院2008年11月至2009年11月入院进行治疗的腹水患者25例,其中男性患者16例,女性患者9例,患者年龄在18～81岁,中位年龄为56岁。运用流式细胞检测法对腹水DNA含量进行测定与鉴别。结果恶性腹水中癌细胞的检出率只有40%左右,而且细胞病理学的采样工作需要重复进行许多次,并且检测者的主观因素会对检测结果产生一定的影响。结论联合应用腹水DNA含量对良恶性腹水有良好效果,值得推广。Objective Determination of DNA ascites differentiating benign from malignant ascites method for the diagnosis of cancer research.Methods A randomly selected in the Second Affiliated Hospital of Baotou Medical College from November 2008 to November 2009 for treatment of ascites admitted 25 patients, 16 patients were male, 9 cases of female patients, patients aged 18 to 81 years old, with a median age 56.Detection method using flow cytometry DNA content of ascites were measured and identified.Results Malignant ascites in cancer detection rate of only about 40%, and cellular pathology of sampling need to be repeated many times, and the detection of the subjective factors will have some impact test results.Conclusion The combination of DNA content in benign and malignant ascites has a positive effect, it is worth promoting.

关 键 词：腹水 DNA含量 鉴别 

分 类 号：R442.5[医药卫生—诊断学]