PCR杂交梳技术在乙型肝炎诊断中的应用  

Application for Detecting Hepatitis B Virus with Hybri-Comb Technique

作　　者：郭劲松[1] 曾令兰[1] 李淑莉[1] 罗端德[1] 李璐 

机构地区：[1]同济医科大学附属协和医院传染病教研室,武汉430022

出　　处：《中西医结合肝病杂志》1999年第3期8-9,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:为临床诊断乙型肝炎提供灵敏、准确、可靠的实验依据。方法:采用PCR杂交梳(反相膜杂交法),对71例乙型肝炎患者血清进行DNA验证性检测,并与一般聚合酶链反应(PCR琼脂糖凝胶电泳法)同步对比分析。结果:71例乙型肝炎患者血清中杂交梳方法检出HBV DNA阳性58例,阳性率81.7％;琼脂糖凝胶电泳质检出HBV DNA阳性50例,阳性率70％。结论:PCR杂交梳法较PCR法具有更高特异性和敏感性。Aim:To probe into a more sensitive.more accurate and more specific method to detect HBV DNA. Methods: Serum samples of 71 patients with hepatitis B were detected for HBV DNA by PCR hybri-comb (reversal membrane hybridization), at the same time, usual polymerase chain reaction assay (PCR-electrophoresis in agarose gel) was given as controls. Result: 58 serum samples were positive for HBV DNA by PCR hybri-comb assay among 71 patients samples. Positive ratio was 81. 7% ; however, 50 serum samples were positive by PCR-electrophoresis in agarose gel, and positive ratio was 70%. Conclusion: PCR hybri-comb technique is satisfactory with sensitivity and specificity in detecting HBV DNA.

关 键 词：聚合酶链反应 乙型肝炎 诊断 

分 类 号：R512.620.4[医药卫生—内科学]