大鼠脾脏收缩功能与α肾上腺素受体亚型的调节  

Relationship between adrenoceptor subtypes and contraction of rat spleen

作　　者：保庭毅[1,2] 何泽生[1,2] 吴金生[1,2] 臧益民[1,2] 牛国保[1,2] 

机构地区：[1]第四军医大学唐都医院普通外科 [2]第四军医大学生理学教研室

出　　处：《心功能杂志》1999年第2期95-96,99,共3页

摘　　要：目的：探讨脾脏收缩功能与α肾上腺素受体（α－ＡＲ）及亚型分布的关系。方法：采用药物受体分析方法，测定应用拮抗剂前后，ｐＡ２，ｐＤ２，ＥＣ５０，Ｒｍａｘ的值。结果：①对Ｒｍａｘ的抑制：硝苯吡啶（Ｎｉｆ）＋育亨宾（Ｙｏｈ，４３％）＞哌唑嗪（Ｐｒａｚ，３９％）＞酚妥拉明（Ｐｈｅｎ，２７％）＞Ｙｏｈ（１９％）；②脾脏ＮＥ累积浓度收缩曲线右移程度（在ＥＣ５０水平），Ｐｒａｚ＝Ｎｉｆ＋Ｙｏｈ（３．４倍）＞Ｐｈｅｎ（３．０倍）＞Ｙｏｈ（１．７倍）；③各组均能显著降低ｐＤ２；④ｐＡ２：Ｐｒａｚ，Ｐｈｅｎ，Ｙｏｈ三者间无差异，但它们均显著高于Ｎｉｆ＋Ｙｏｈ组。结论：①大鼠脾脏α受体亚型为α１，α２，且α１略多于α２亚型。②大鼠脾脏α１受体亚型介导收缩效应并不依赖于细胞外Ｃａ２＋的存在。可能α１亚型为α１Ｂ亚型。Aim:To investigate the relationship between adrenoceptor subtypes and contraction of rat spleen Methods:According to the effect after agonist or antagonist on NE dose response of preparations,the pA2,pD2,EC50 and Rmax were calculated by using pharmacological methods Z]Results: The suppression of Rmax;Mif+Yoh(43%)>Praz(39%)>Phen(27%)>Yoh(19%);NE dose response curves were shifted to the right (at EC50 level)Praz=Nif+Yoh(3.4 fold)>Phen(30 fold)>Yoh(17 fold);The pD2 were reduced by each groups;PrazPhenYoh(P>005) but they were higher than Nif+Yoh group Conclusion: In spleen of rat,the distribulion of AR subtypes were both 1 type and 2 type, 1>2,The contractile responses induced by 1 subtypes were not depended on intracellular Ca2+ The 1 subtypes were 1B subtypes probably

关 键 词：受体 肾上腺素能 亚型 脾脏 

分 类 号：R331.21[医药卫生—人体生理学] Q577[医药卫生—基础医学]