皮质醇对牛蛙心肌收缩的快速作用  

Rapid effect of cortisol on myocardial contractility in bullfrog

作　　者：郝怡鑫[1] 王建文[1] 邢宝仁[1] 

机构地区：[1]第二军医大学生理学教研室

出　　处：《心功能杂志》1999年第2期100-101,105,共3页

摘　　要：本实验采用八木氏法灌流牛蛙心脏，经换能器、放大器输入计算机记录心肌的收缩活动，观察皮质醇对牛蛙心肌收缩的作用。结果如下：①灌流１×１０－６ｍｏｌ／Ｌ皮质醇，蛙心收缩幅度增大，潜伏期平均为１８．７ｓ，蛙心收缩频率无明显改变（ｎ＝６０）。②灌流２×１０－７ｍｏｌ／Ｌ～１×１０－６ｍｏｌ／Ｌ不同浓度皮质醇后，随着皮质醇浓度的增加，心肌收缩幅度也增大，表现明显的量效关系（ｎ＝２０）。③甾体激素胞内受体阻断剂—１×１０－６ｍｏｌ／ＬＲＵ３８４８６不能阻断皮质醇的作用（ｎ＝２０）。④经蛋白合成抑制剂—放线菌酮作用２０ｍｉｎ后，再灌流１×１０－６ｍｏｌ／Ｌ皮质醇，心肌收缩幅度仍增大（ｎ＝２０）。上述结果提示：皮质醇对牛蛙心肌收缩具有快速增强作用。The effect of cortisol on myocardial contractility in bullfrog were studied with the BAMUHARTUNGS method The results are as follows:The magnitude of myocardial contractility was increased when the heart of bullforg was perfused with 110-6 mol/L cortisol,the latency was 18723 second(n=60) In the concentration range of 210-7 mol/L to 110-6 mol/L of cortisol, the effect of cortisol was concentration dependent The perfusion of 110-6 mol/L RU38486 has no significant influence on the responsce of myocardial contratility to 110-6 mol/L cortisol(n=20) The potentiation of myocardial contractility by 110-6 mol/L cortisol did not diminished when the preparation was preincubated in the presence of actidione for twenty minutes (n=20) This results indicate that the cortisol has the rapid excitatory effect on the myocardial contractility of bullfrog

关 键 词：皮质醇 心肌收缩 牛蛙 

分 类 号：Q463[生物学—生理学]