HPLC法测定不同来源甘草中甘草酸的含量  

Content Determination of Glycyrrhizic Acid in Glycyrrhiza uralensis from Different Sources by HPLC

作　　者：唐丽琴[1] 张善堂[1] 刘圣[1] 石允卉 

机构地区：[1]安徽医科大学附属省立医院药剂科,合肥市230001

出　　处：《中国药房》2010年第39期3700-3701,共2页China Pharmacy

基　　金：安徽省自然科学基金面上项目(090413106)

摘　　要：目的:建立以高效液相色谱法测定甘草中有效成分甘草酸含量的方法,并测定不同产地和基原甘草药材中甘草酸的含量。方法:色谱柱为Hypersil-C18(250mm×4.6mm,5μm),流动相为甲醇-0.2mo·lL-1醋酸铵溶液-冰醋酸-三乙胺(64:36:1.5:0.02),流速为1.0mL·min-1,检测波长为252nm,柱温为30℃。结果:甘草酸检测浓度在0.0416～0.2080mg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为101.3%,RSD=1.22%(n=9)。结论:不同来源甘草药材中甘草酸的含量差异较大,野生甘草中甘草酸的含量高于家种。OBJECTIVE：To establish the HPLC method for the content determination of glycyrrhizic acid in Glycyrrhiza uralensis from different sources.METHODS：The separation was performed on Hypersil-C18（250 mm×4.6 mm,5 μm）column with mobile phase consisted of methanol-0.2 mol·L^-1 ammonium acetate-glacial acetic acid-triethylamine（64：36：1.5：0.02） with the flow rate of 1.0 mL·min-1.The detection wavelength was set at 252 nm and column temperature was 30 ℃.RESULTS：The linear range of glycyrrhizic acid was 0.041 6～0.208 0 mg·mL^-1（r=0.999 9） with an average recovery of 101.3%（RSD=1.22%,n=9）.CONCLUSION：The content of glycyrrhizic acid is variant in different origin and sources.The content of glycyrrhizic acid in wild G.uralensis is higher than that of domestic species.

关 键 词：甘草 甘草酸 高效液相色谱法 含量 来源 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]