黄斑颗粒含药血清预处理对人RPE细胞缺氧损伤的保护作用  

Protective Effects of Pretreatment with Drug Serum of "Huangban Granule" on Hypoxic Injury of Human Retinal Pigment Epithelial Cell

作　　者：刘晓娟[1] 宫媛媛[1] 吴星伟[1] 顾青[1] 

机构地区：[1]上海交通大学附属第一人民医院眼科,上海200080

出　　处：《上海中医药大学学报》2010年第5期73-76,共4页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市卫生局中医药重大课题基金资助项目(2006ZD01)

摘　　要：目的:观察中药黄斑颗粒含药血清预处理后对人视网膜色素上皮(hRPE)细胞缺氧损伤的保护作用。方法:制备不同剂量中药黄斑颗粒含药血清,用含药血清预处理hRPE细胞,分不同时间段建立化学缺氧损伤模型,培养24h后,用MTT法检测细胞活性,流式细胞仪(FCM)检测细胞周期。结果:黄斑颗粒含药血清组细胞活力较空白血清组与模型组细胞活力显著提高,其中高剂量含药血清组细胞活力高于低剂量含药血清组,但仍低于正常对照组,有显著性差异;各含药血清组细胞活力抑制率均明显降低,其中高剂量组低于低剂量组。此外,含药血清能促进缺氧条件下RPE细胞由G0/G1期进入S期,促进RPE细胞的增殖。结论:中药黄斑颗粒含药血清预处理对人视网膜色素上皮细胞缺氧损伤具有一定的保护作用。Objective：To observe the protective effects of pretreatment with drug serum of ＂Huangban Granule＂ on hypoxic injury of human retinal pigment epithelial （hRPE） cells. Methods： Different dosages of drug serum of ＂Huangban Granule＂ were prepared and the model of hypoxic injury of hRPE cells were established at different time. The hRPE cells were pretreated by drug serum and the cell activity and cell cycle were detected by MTT method and flow cytometer respectively after 24 hours. Results： The cell activity in drug serum groups was higher than that in control group and model group. The cell activity in high dosage drug serum group was higher than that in low dosage drug serum group but lower than that in normal group. The inhibitory rates of cell activity in drug serum groups were decreased significantly and the inhibitory rates of cell activity in high dosage drug serum group were lower than those in low dosage drug serum group. In addition the drug serum of ＂Huangban Granule＂ can promote RPE cells in the condition of hypoxia from G0/G1 phase to S phase and promote the proliferation of RPE cells. Conclusion： The pretreatment with drug serum of ＂Huangban Granule＂ has protective effects on hypoxic injury of hRPE cells.

关 键 词：“黄斑颗粒” 人视网膜色素上皮细胞 缺氧 保护作用 

分 类 号：R285.5[医药卫生—中药学]